Spatiotemporal control of CRISPR/Cas9 function in cells and zebrafish using light-activated guide RNA.

Answers

Answer 1

"Spatiotemporal control of CRISPR/Cas9 function in cells and zebrafish using light-activated guide RNA."

This study focuses on utilizing light-activated guide RNA (gRNA) to control the function of the CRISPR/Cas9 gene-editing system in cells and zebrafish. The researchers developed a system where gRNAs can be activated by light, allowing precise spatial and temporal control over the gene editing process. This technology enables researchers to precisely target and modify specific genes in living organisms, providing a powerful tool for studying gene function and potentially treating genetic diseases. The study demonstrates the potential of light-activated gRNA for enhancing the precision and versatility of the CRISPR/Cas9 system.

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Related Questions

procleave: predicting protease-specific substrate cleavage sites by combining sequence and structural information. genomics proteomics bioinformatics

Answers

Procleave is a bioinformatics tool that combines sequence and structural information to predict protease-specific substrate cleavage sites. It has applications in genomics, proteomics, and bioinformatics, allowing researchers to gain insights into protein function and cellular processes.

"procleave" is a bioinformatics tool that predicts protease-specific substrate cleavage sites by combining sequence and structural information. It is used in the field of genomics, proteomics, and bioinformatics.
To predict substrate cleavage sites, procleave analyzes both the sequence and structure of a protein. It takes into account specific patterns or motifs that are recognized by different proteases. These patterns can be identified by analyzing the amino acid sequence of a protein and comparing it to known cleavage sites for a particular protease.
The tool also considers the structural information of the protein, such as the presence of specific secondary structures like alpha helices or beta sheets. By combining both sequence and structural information, procleave improves the accuracy of predicting protease-specific substrate cleavage sites.
In genomics, the study of an organism's complete set of genes, tools like procleave can be useful in understanding how proteases function and how they contribute to various cellular processes. Proteases play important roles in protein degradation, signal transduction, and regulation of gene expression. By predicting protease cleavage sites, researchers can gain insights into protein function and design experiments to investigate their biological roles.
In summary, procleave is a bioinformatics tool that combines sequence and structural information to predict protease-specific substrate cleavage sites. It has applications in genomics, proteomics, and bioinformatics, allowing researchers to gain insights into protein function and cellular processes.

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Which enzyme works best in neutral conditions?

Answers

Pepsin purpose at the lowest pH. Pepsin is an enzyme that works in the stomach to digest proteins from foods we consume.

Which enzyme works best at neutral pH?

Pepsin has a best of pH 2.5 and a working range between pH 1-4. Catalase has an optimum pH of 9 and a working range of linking pH 7-11. Most other enzymes function within an employed pH range of about pH 5-9 with neutral pH 7 being the optimum.

Enzymes are affected by a swap in pH. The most favorable pH value - the point where the enzyme is most active - is known as the optimum pH.

So we can conclude that pepsin works in highly acidic state of the stomach. Its enzyme has an optimum pH of about 1.5.

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A leading fruit juice manufacturing company wants to launch a new flavor in the market. For this, the company wants Information about the latest consumer trends and tastes. How will the management team acquire information about the latest consumer trends and tastes?

A. by understanding group dynamics
B. by exercising leadership qualities
C. through the organizing function
D. through market research​

Answers

Answer:

D. through market research​

Explanation:

Market research can be defined as a strategic technique which typically involves the process of identifying, acquiring and analyzing informations about a business. It involves the use of product test, surveys, questionnaire, focus groups, interviews, etc.

Secondary market research can be defined as a method designed to determine the demographics of a particular target market.

In this scenario, a leading fruit juice manufacturing company wants to launch a new flavor in the market. For this, the company wants Information about the latest consumer trends and tastes. Thus, in order for the management team to acquire information about the latest consumer trends and tastes, they should conduct a market research.

in plants the fertilized egg develops into a multicellular _________ within a female gametangium.

Answers

Answer:

Once fertilization has occurred, the embryo develops within the female gametophyte, and the ovule becomes the seed, complete with a food source (the gametophyte tissue) and a seed coat (the integument).

Give four functions of the mouth in the digestive process

Answers

Answer:

receive food by ingestionbreak it into small particles by masticationmix it with salivamoistens food

Answer:

The four functions of the mouth in the digestive process is given below:

Explanation:

1. Digestive process starts from mouth.

2. It moistens food so it move more easily through esophagus into stomach.

3. Mouth produces saliva which has an enzyme that begins to break down starches in food.

4. Mouth chews food and digestion involves breaking down the food into simpler nutrients that can be used by cells.

in the griffiths-avery-mccarty experiments, how was it determined that it was dna that transformed rough bacteria into smooth?

Answers

Answer: In the Griffiths-Avery-McCarty experiments, it was determined that it was DNA that transformed rough bacteria into smooth by the experiments of Oswald Avery and his colleagues.

Avery, Colin MacLeod, and Maclyn McCarty's experiments were based on Griffith's finding that R strain bacteria could be transformed into S strain bacteria by dead heat-killed S strain bacteria. It was important to establish the mechanism of transformation, as well as the substance that carried the genetic material responsible for the transformation.

To achieve this goal, Avery, MacLeod, and McCarty executed a series of experiments that were designed to identify the material responsible for the transformation. They first divided the S strain into its main biochemical components, including RNA, DNA, proteins, and lipids, and then treated the R strain with each of these components. The transformation only occurred when the R strain was treated with DNA.

The R strain, on the other hand, did not transform when treated with RNA, protein, or lipid. The researchers came to the conclusion that DNA is the hereditary substance that transmits genetic information from one generation of organisms to the next. This discovery altered the study of genetics and advanced research on DNA's function and structure.



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Can someone help me

Can someone help me

Answers

Dominant, recessive, heterozygous, dominant

explain the primary differences between artificial and natural selection.

Answers

Answer:

Natural selection is created biological and artificial are human-made process.

Explanation:

What is the opposite of precipitation?

Answers

Dissolution plz give brainlyest

Answer:

Disssolution

Explanation:

Precipitation is the process of a compound coming out of solution. It is the opposite of dissolution or solvation. In dissolution, the solute particles separate from each other and are surrounded by solvent molecules. In precipitation, the solute particles find each other and form a solid together


.



What is maintaining constant internal conditions?

Answers

Answer:

Homeostasis

Explanation:

just search online lol

the greatest single cause of death among cigarette smokers is ______.

Answers

The greatest single cause of death among cigarette smokers is cardiovascular disease.

Cigarette smoking is a major risk factor for developing various cardiovascular conditions, including heart disease, stroke, and peripheral vascular disease.

The harmful chemicals in tobacco smoke, such as nicotine and carbon monoxide, can damage the cardiovascular system in multiple ways.

Nicotine causes blood vessels to constrict and raises blood pressure, increasing the workload on the heart.

Carbon monoxide reduces the amount of oxygen that can be carried by the blood, leading to oxygen deprivation in various organs, including the heart and brain.

Over time, smoking can lead to the development of atherosclerosis, which is the buildup of plaque inside the arteries.

This narrows the arteries and restricts blood flow, increasing the risk of heart attacks and strokes.

In conclusion, cardiovascular disease is the leading cause of death among cigarette smokers due to the detrimental effects of smoking on the heart and blood vessels.

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According to the theory, which antibody isotype crosses the placenta? A) IgA. B) IgM. C) None. D) IgG.

Answers

According to the theory, the antibody isotype that crosses the placenta is IgG. The answer is option D)

During pregnancy, IgG antibodies produced by the mother can cross the placenta and enter the fetal circulation, providing protection against certain infectious diseases that the mother has been exposed to in the past.

This transfer of maternal antibodies to the fetus is particularly important in the first few months of life when the infant's immune system is not yet fully developed.

In contrast, IgA antibodies are mainly found in mucosal secretions and do not cross the placenta, while IgM antibodies are too large to cross the placental barrier.

Therefore, the correct answer is D).

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Suppose that instead of consuming small fish, the mackerel begins to consume small amounts of leftover plastic polluting the ocean. Describe the impact that this would have on the Mackerel, Snapper Fish and Large Shark populations. Explain your reasoning.

Suppose that instead of consuming small fish, the mackerel begins to consume small amounts of leftover

Answers

Answer:

It creates a bigger food chain

Explanation:

The density of the material will ___ when thermal energy is added to it.​

Answers

Answer:

the unit used for measuring the heat.

Explanation:

depending on the work your doing it's D/4

A population of water beetle lives in a small pond on a cattle range. During a severe drought, a herd of cattle accidentally drink up many water beetles from a water source, preventing the beetle's genes from being passed to future generations. Which of the following evolutionary mechanisms is represented by this scenario?
A.Gene Flow
B.Genetic Drift
C.Independent Assortment
D.Mutation

Answers

I believe the answer is A


Base your answer on the diagram below which represents a portion of a double-stranded
DNA molecule and on your knowledge of biology.

The Symbol labeled 3 represents a molecule of a

A) 5-carbon sugar
B) 6-carbon sugar
C) 3-carbon sugar
D) 4-carbon sugar

Base your answer on the diagram below which represents a portion of a double-strandedDNA molecule and

Answers

Answer:

A

Explanation:

Each nucleotide in a DNA sequence is made up of:

A pentose (5 carbon) sugar 1 nitrogenous base1 phosphate group

The pentose sugar is even shaped like a pentagon, so when in doubt simply count the sides!

Hope this helped you today :)

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help me on theis pls pls pls

Answers

Answer:

1. D

2. B

3. C

4. A

5. False

6. True

7. False

Explanation:

what is the average recycling rate of the world.

Answers

Answer:

32.1 percent

The recycling rate (including composting) was 32.1 percent in 2018, down from 34.7 percent in 2015. The per capita rates in 2018 were: 1.16 pounds per person per day for recycling.

Explanation:

Answer:

97 % but am not 100% sure

Explanation:

in humans there may be three times as many proteins as genes. if each gene encodes a protein, how can there be more proteins than genes?

Answers

In humans, there are approximately 20,000-25,000 protein-coding genes, but the estimated number of proteins is around 100,000-500,000. This disparity is due to a process called alternative splicing and post-translational modifications.

Alternative Splicing: During the formation of messenger RNA (mRNA) from a gene, the introns (non-coding regions) are removed and the exons (coding regions) are spliced together. Alternative splicing is when different combinations of exons are joined together, creating multiple mRNA molecules from a single gene. Each mRNA molecule can then be translated into a unique protein, increasing the diversity of proteins produced by a single gene.Post-Translational Modifications: After a protein is synthesized, it can undergo further modifications such as phosphorylation, glycosylation, or acetylation. These modifications can change the structure and function of the protein, allowing a single gene to encode for multiple protein variants with distinct properties.In summary, the reason humans have more proteins than genes is due to the complexity of gene expression through alternative splicing and post-translational modifications. These processes expand the diversity of proteins and their functions in the human body, despite the limited number of protein-coding genes.

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The nitrogenous bases were examined in a specific sequence of DNA that codes for a
gene that creates a protein. 70% of the DNA was adenine. What percent of the DNA
would you expect the scientist to find for thymine in that same genetic sequence?
А
0%
B
25%
с
30%
D
70%

Answers

D 70%. Adenine pairs with thymine so if there was 70% of adenine it would match and vice versa. Same goes with guanine and cytosine

In the given DNA sequence, the percentage of the DNA would you expect the scientist to find for thymine in that same genetic sequence - D. 70%

Chargaff's rules

The DNA from any cell of all organisms should have a 1:1 ratio of purine which is cytosine, thymine and pyrimidine bases which guanine and adenine.

As we know Adenine always binds with thymine in DNA then there would be always equal in each sample or sequence. Thus, the the percentage of the DNA would you expect the scientist to find for thymine in that same genetic sequence would be 70%.

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Which two of the three elements have SIMILAR CHEMICAL PROPERTIES (valence electrons)?
1. Li - Na - Mg
2. F - Br - O
3. P - As - Se
4. Ra - Ba - Cs

Answers

Answer:

1. Li - Na

2. F - Br

3. P - As

4. Ra - Ba

How do the results of the simulations in Lessons 10 and 11 help you explain how it is possible that applying antibiotics can lead to a population that becomes more resistant to antibiotics than they were initially, even when individual bacterium traits are not changing and the trait variations that an offspring inherits are identical to those of its parent?

Answers

Answer:

its like cov/d. if u get it you will be ammune when it goes away. so its the same wit this situation.

Explanation:

________is the movement of water from an area of high concentration to an area
of low concentration.
Exocytosis
Osmosis
Diffusion
Endocytosis

Answers

The answer is Diffusion
The answer is diffusion.
hope this helps x

Chromosomes contain genes, and genes determine an organism's characteristics. Sometimes mutations occur in which the sequence of nucleotides within a gene becomes altered. Which of the following describes how a gene mutation would most likely affect an organism?A.It would cause different proteins to be produced during translation.B.It would cause the sugar-phosphate backbone of all of the organism's DNA to be modified.C.It would cause the codons produced to be more or less than 3 nucletoides in length.D.It would cause the wrong type of nucleic acid to be incorporated into the organism's genes.

Answers

A gene mutation would most likely affect an organism by A. causing different proteins to be produced during translation.

Genes contain the instructions for building proteins, and mutations can alter the sequence of nucleotides within a gene. The sequence of nucleotides determines the sequence of amino acids in a protein, which, in turn, determines its structure and function.When a mutation occurs in a gene, it can result in changes to the mRNA molecule transcribed from the gene. These changes can lead to different codons being produced during translation, which can cause substitutions, insertions, or deletions of amino acids in the resulting protein. As a result, the protein's structure and function may be altered, leading to changes in the organism's characteristics or traits. It's important to note that not all mutations have noticeable effects on an organism, and the impact of a mutation depends on its location within the gene, the type of mutation, and the specific protein that is affected.

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Which is the main cell triggered by electrical impulses to send chemical messages in the brain?.

Answers

Your answer would be the neuron :)

what is the net of veins and arteries that connect the respiratory system with the circulatory system together?

Answers

the answer is capillaries

Which process is included in the formation of both metamorphic and sedimentary rock?
A.
heating
B.
recrystallization
C.
compaction
D.
weathering

Answers

Answer:

C.  compaction

Explanation:

The weight on the lower layers increases as the layers of sediment stack up. The soils are pressed together, squeezing out some water mixing in with the sediments. This approach is known as compaction.

The sedimentary series has formed into a sedimentary stone following compaction and cementation.

What is a major source of energy for cellular processes in both plants and animals

Answers

The ultimate source of energy for all living things is the sun. Plants convert light energy to chemical energy in photosynthesis. Animals obtain energy by eating plants. The only form of energy a cell can use is a molecule called adenosine triphosphate (ATP).


In 1988, James Hansen projected future warming trends. He used 3 different scenarios, identified as A, B, and C. Each represented
different levels of greenhouse gas emissions. All three indicated a strong cause and effect relationship between observed
temperatures and human emissions of greenhouse gases into the atmosphere. Greenhouse gas emissions are at the center of
almost all climate change concerns. What are the likely effects of increasing Greenhouse gas emissions on the ecosystem? Select
ALL that apply.
A)
rising sea-levels and ocean acidification
B)
changes in precipitation resulting in flooding and droughts
more extreme, erratic, unpredictable weather, including intense hurricanes
D)
decreases in respiratory illnesses due to the warming temperatures and
shorter winters
E)
increases in agriculture production due to increased CO2 availability for
photosynthesis

Answers

Answer: a b c

Explanation: lol I just got it wrong so ik the answers

A) rising sea-levels and ocean acidification

B) changes in precipitation resulting in flooding and droughts

C) more extreme, erratic, unpredictable weather, including intense hurricanes

What is green house effect?

The greenhouse effect is a natural process that occurs when certain gases in the Earth's atmosphere, such as carbon dioxide, methane, and water vapor, trap heat from the sun. These gases act like a blanket, keeping the Earth's surface warm enough to sustain life. Without the greenhouse effect, the Earth would be too cold to support most forms of life.

However, human activities such as burning fossil fuels have increased the levels of greenhouse gases in the atmosphere, leading to an enhanced greenhouse effect and global warming. This is a significant environmental issue as it can cause harmful impacts on ecosystems and human societies.

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HELP ASAP PLSS

1.How can we trace the lines of descent between species?


Answers

Looking at the features of past species you can determine how old and what differences they have from fossils.

We can trace the lines of descent between species by three methods that are comparative anatomy, comparative embryology, and molecular genetics.

What is phylogenetics?

Phylogenetics is the study of evolutionary relationships among biological entities – often species, individuals or genes (which may be referred to as taxa).

Thus, We can trace the lines of descent between species by three methods that are comparative anatomy, comparative embryology, and molecular genetics.

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