When you have completed your PCR, you will be visualizing the fragments on an agarose gel. You should obtain many copies of a single fragment of approximately 1000 base pairs. Instead, you find three fragments: one of 1,000, one of 850, and one of approximately 600 base pairs. What could you do next time to decrease the number of bands that you obtain?

heh.......... sorry man............... but this was posted over 5 hours ago........ so nobody is gonna see it and you probably dont need the answer anymore..... so errrr..... imma justtttt..... take these points :D

Answer:

see explanation

Explanation:

plants and animals have a symbiotic relation.

animals breathe in oxygen. they eat plants directly or they eat other animals that eat plants.

the plants contain carbon-water molecules that are called carbo-hydrates, which also contain energy from

sunlight. Animals breathe oxygen. the oxygen reacts with the carbo-hydrate to release the water and make carbon dioxide, both of which animals breathe out when they have respiration

the plants bbreath in the carbon dioxide and react it with water and sunlight using chlorophyll, storing the sun energy in the carbo-hydrate and breathing out oxygen in their respiration.

plants need animals for their carbon dioxide, animals need plants for their carbohydrates and for the

oxygen they breathe out.

the plants breath in the carbon

Answer:

the sing post

Explanation:

because the reference frame is a fixed object that determines whether a body is in motion or not so the other passengers are fixed but relative to the the the man is at rest but relative to the sign post it is in the motion, did I get me, sister

Answer:

17 g

Explanation:

The number of calories required for 47.5g of Al to go from 25 to 62 is 3,689.9 cal.

Calories are a measure of energy. They are the amount of energy that is released when food is digested, broken down and converted into energy for the body to use. The energy from calories is used to fuel physical activities, as well as all the bodily functions that keep us alive. Eating food is the main source of calories, although some drinks also contain calories. Different types of food contain different amounts of calories. Foods that are high in fat and sugar tend to contain more calories.

Calories (cal) = Heat (J) / 4.184
Heat (J) = Mass (g) x Specific Heat (J/g C) x Change in Temperature (C)
Heat (J) = 47.5g x 0.900 J/g C x (62 - 25) C
Heat (J) = 15,495 J
Calories (cal) = 15,495 J / 4.184
Calories (cal) = 3,689.9 cal.

To learn more about calories
https://brainly.com/question/28339171
#SPJ1

Answer:

The correct option is;

Scientists can drill deep into the ice to collect ice cores which contain trapped atmospheric gases

Explanation:

The study of past climates also known as paleoclimatology, is accomplished by acquiring information from proxy data sources which are physical environment characteristics that are preserved through time to remake the conditions of past climate

Past physical environmental characteristics, from which information about ancient climate can be gained are stored in nature's climate variability records including, ice cores, rings in tree stems, fossil pollen, sediments found in the waters of the ocean

The proxy sources provide a means of understanding the conditions of ancient climate before advent of climate measurement.

Therefore, one way that analyzing ice benefits scientists who study ancient climates is that scientists can drill deep into the ice to collect ice cores which contain trapped atmospheric gases from past climates.

Answer: B: Scientists can study the layers of ice cores to gather information about past atmospheric composition.

Explanation: Got it right on a test!

The fact that coal releases more heat than charcoal means that the chemical potential energy is lower in charcoal than in coal.

When the substance is burnt in oxygen, the chemical potential energy stored in the substance is converted into heat energy. The substance that has the most chemical potential energy releases the most energy when burnt.

In burning coal and charcoal, you notice that coal releases more heat energy than charcoal. This means that the chemical potential energy is lower in charcoal than in coal.

Learn more; https://brainly.com/question/22220547

Answer:

a)Carbon (iv) oxide; CO₂

b)It is used in fire extinguishers

c) i. It is denser than air

ii. It dissolves in water to form an acidic solution

Explanation:

a) Since the compound P is composed of one atom of carbon and two atoms of oxygen, the formula of the compound is CO₂. The name of the compound is carbon(iv) oxide.

b) Carbon (iv) oxide is a gas that is of great importance to living organisms. Plants require it for photosynthesis while human produce it during the process of respiration.

Industrially, it is used in carbonated drinks. It is also used in fire extinguishers

c) The properties of Carbon (iv) oxide include the following:

i) it is an odourless colorless gas

ii) it is denser than air

iii) it dissolves in water to form a weak acidic solution, etc.

Answer: The number of moles of excess reagent left is 0.750 moles

Explanation:

The number of moles is calculated by using the equation:

\(\text{Number of moles}=\frac{\text{Given mass}}{\text{Molar mass}}\) ......(1)

Given mass of \(KI\) = 249 g

Molar mass of \(KI\) = 166 g/mol

Putting values in equation 1, we get:

\(\text{Moles of KI}=\frac{249g}{166g/mol}=1.5mol\)

Given mass of \(Pb(NO_3)_2\) = 496.5 g

Molar mass of \(Pb(NO_3)_2\) = 331 g/mol

Putting values in equation 1, we get:

\(\text{Moles of }Pb(NO_3)_2=\frac{496.5g}{331g/mol}=1.5mol\)

The given chemical equation follows:

\(2KI+Pb(NO_3)_2\rightarrow 2KNO_3+PbI_2\)

By stoichiometry of the reaction:

If 2 moles of KI reacts with 1 mole of lead(II) nitrate

So, 1.5 moles of KI will react with = \(\frac{1}{2}\times 1.5=0.75mol\) of lead(II) nitrate

As the given amount of lead(II) nitrate is more than the required amount. Thus, it is present in excess and is considered as an excess reagent.

Thus, KI is considered a limiting reagent because it limits the formation of the product.

Moles of excess reactant (\(Pb(NO_3)_2\)) left = [1.5 - 0.75] = 0.750 moles

Hence, the number of moles of excess reagent left is 0.750 moles

The amount of heat energy required to melt the 182 g of ice at -18 ˚C to liquid water at temperature of 25 ˚C is 86475.48 J

To obtain the heat energy required, do the following:

1. Obtain the heat required to change the ice from -18 ˚C to 0 °C. This is shown below:

Q₁ = MCΔT

Q₁ = 182 × 2.030 × 18

Q₁ = 6650.28 J

2. Obtain the heat required to melt the ice. This is shown below:

Q₂ = m × ΔHf

Q₂ = 182 × 334

Q₂ = 60788 J

3. Obtain the heat required to change the water from 0 °C to 25 °C. This is shown below:

Q₃ = MCΔT

Q₃ = 182 × 4.184 × 25

Q₃ = 19037.2 J

4.  Combine the heat energies obtained above to obtain the heat required to melt the ice from -18 ˚C to water at 25 ˚C. This is shown below:

Q = Q₁ + Q₂ + Q₃

Q = 6650.28 + 60788 + 19037.2

Total heat energy required = 86475.48 J

Learn more about heat transfer:

https://brainly.com/question/25878495

#SPJ1

The elements in hydroxyapatite, a major compound in human bones and teeth, are calcium, phosphorus, oxygen, and hydrogen.

Hydroxyapatite (OH) is a major compound found in human bones and teeth. It is primarily composed of the elements calcium (Ca), phosphorus (P), oxygen (O), and hydrogen (H). The hydroxyapatite formula (OH) indicates the presence of a hydroxyl group (OH-) within the compound.

To determine the elements in hydroxyapatite, we break down the compound into its constituent elements:

Calcium (Ca) is an essential component, contributing to the structure and strength of bones and teeth.

Phosphorus (P) is also a crucial element in hydroxyapatite, playing a role in bone mineralization and providing rigidity.

Oxygen (O) is present in hydroxyapatite as it forms strong bonds with calcium and phosphorus, contributing to the overall stability of the compound.

Hydrogen (H) is part of the hydroxyl group (OH-) in hydroxyapatite, which enhances the compound's chemical properties.

Therefore, the elements present in hydroxyapatite, a major compound in human bones and teeth, are calcium (Ca), phosphorus (P), oxygen (O), and hydrogen (H).

Learn more about hydroxyapatite from the given link: https://brainly.com/question/31444356

#SPJ11

Answer:

The Greeks invented the two main components of watermills, the waterwheel and toothed gearing, and used, along with the Romans, undershot, overshot and breastshot waterwheel mills.

Explanation:

please mark me brainliest

Answer:

they will eat a lot of fish and make other animals go hungry

Explanation:

logic

As the population of large predator fish has grown out of control it will require more supply food due to which population of small fishes will decrease leading to disturbance in ecosystem.

Ecosystem is defined as a system which consists of all living organisms and the physical components with which the living beings interact. The abiotic and biotic components are linked to each other through nutrient cycles and flow of energy.

Energy enters the system through the process of photosynthesis .Animals play an important role in transfer of energy as they feed on each other.As a result of this transfer of matter and energy takes place through the system .Living organisms also influence the quantity of biomass present.By decomposition of dead plants and animals by microbes nutrients are released back in to the soil.

Learn more about ecosystem,here:

https://brainly.com/question/19267717

#SPJ2

Answer:

(CH3COO)2 + redP + Cl2 → ClCH2COOH + HCl

Explanation:

This is an example of halogenation of carboxylic acids at alpha carbon atom. In this reaction, red phosphorus and chlorine are treated with carboxylic acids having alpha hydrogen atom followed by hydrolysis to form alpha chloro carboxylic acid.

Answer:

solubility

Explanation:

the solid substances will be added to water to see which one is soluble

The property of matter that is conserved in chemical reactions and shown by balanced equations is known as the Law of Conservation of Mass. According to this law, mass can neither be created nor destroyed in a chemical reaction; it can only be transformed from one form to another.For instance, when two substances are combined, they react and form a new substance.

For such more question on chemical reaction

https://brainly.com/question/11231920

#SPJ8

Answer: D. Investigation 4

Answer:

Investigation D

Explanation:

When classifying p and d orbital diagrams, check if each orbital receives one electron before any pairing occurs to determine if they obey or violate Hunds rule.

To classify p and d orbital diagrams based on whether they obey or violate Hunds rule, you'll first need to understand the rule itself. Hund's rule states that electrons in a subshell will occupy empty orbitals first before pairing up in the same orbital. In other words, each orbital within a subshell will receive one electron before any orbital receives a second electron.

Now, let's consider p and d orbital diagrams.

1. p orbital diagrams: In the p subshell, there are 3 orbitals (px, py, and pz). When filling the p orbitals with electrons, Hund's rule is obeyed if each orbital receives one electron before any pairing occurs. For example:

- px: ↑
- py: ↑
- pz: ↑

This configuration obeys Hund's rule.

A configuration that violates Hund's rule would be:

- px: ↑↓
- py: ↑
- pz:

2. d orbital diagrams: In the d subshell, there are 5 orbitals. When filling the d orbitals with electrons, Hund's rule is obeyed if each orbital receives one electron before any pairing occurs. For example:

- d1: ↑
- d2: ↑
- d3: ↑
- d4: ↑
- d5: ↑

This configuration obeys Hund's rule.

A configuration that violates Hund's rule would be:

- d1: ↑↓
- d2: ↑
- d3: ↑
- d4:
- d5:

So, when classifying p and d orbital diagrams, check if each orbital receives one electron before any pairing occurs to determine if they obey or violate Hund's rule.

Learn more about hunds rule here:
https://brainly.com/question/11862607

#SPJ11

Answer:

Cr(NO3)3 (aq) +K3PO4 (s)

Explanation:

The Kc for the for the given reaction is

Kc = (Kc1Kc2^2) / Kc3 = (6.810^-4 * (3.810^-6)^2) / 1.0910^-8 = 88.5

The given reactions can be combined to obtain the desired reaction as follows:

2HF(aq) + C2O4^2-(aq) <-> 2F-(aq) + H2C2O4(aq)

To find the Kc for this reaction, we can use the principle of chemical equilibrium, which states that the product of the concentrations of the products raised to their stoichiometric coefficients divided by the product of the concentrations of the reactants raised to their stoichiometric coefficients is equal to the equilibrium constant, Kc.

Using this principle, we can express the Kc for the desired reaction in terms of the Kc values for the given reactions as follows:

Kc = (Kc1*Kc2^2) / Kc3

where Kc1 and Kc2 are the equilibrium constants for the dissociation of HF and H2C2O4, respectively, and Kc3 is the equilibrium constant for the desired reaction.

Substituting the given values into this equation, we get:

Kc = (6.810^-4 * (3.810^-6)^2) / 1.09*10^-8 = 88.5

Therefore, the Kc for the desired reaction is 88.5.

For more questions like Equilibrium click the link below:

https://brainly.com/question/31027606

#SPJ11

To find the mass of water in 2.5 litres of the vapor above the solution, we need to calculate the vapor pressure of the solution and then use the ideal gas law.

First, let's calculate the molality of the solution:

molality = mass of solute / mass of solvent

molality = 12.5 g NaCl / (100 g solution - 12.5 g NaCl) = 0.125 mol/kg

Next, we can use the relationship between molality and mol fraction to find the mol fraction of water in the solution:

mol fraction of water = (1 mol/kg) / (1 mol/kg + molality) = (1 mol/kg) / (1 mol/kg + 0.125 mol/kg) = 0.889

Now that we have the mol fraction of water, we can calculate the vapor pressure of the solution:

vapor pressure of solution = mol fraction of water * vapor pressure of pure water = 0.889 * 118 torr = 104.902 torr

Finally, we can use the ideal gas law to find the mass of water in the vapor:

PV = nRT

n = (P * V) / (RT)

n = (104.902 torr * 2.5 L) / (0.0821 Latm/molK * (55 + 273.15) K) = 0.0601 mol

mass of water = n * molar mass of water = 0.0601 mol * 18.015 g/mol = 1.077 g

So, the mass of water in 2.5 liters of the vapor above the solution at 55 C is approximately 1.077 grams.

To know more about ideal gas law, visit the link given below:

https://brainly.com/question/28257995

#SPJ4

The most probable substance that trinity tested is an alkali.

How do you pass a litmus test?

To test at least nine different compounds, cut each piece of litmus paper into three smaller pieces. Take one tiny piece of red litmus paper. Dip it into a sample of the chemical being tested. Put each observation in a proper table. Then put the litmus paper scraps in the trash can.

When the pH is below 4.5 and above 8.3, the litmus paper becomes red. When the paper turns purple, the pH level is almost neutral. If the red paper doesn't change color, the sample is an acid. If the color of the blue paper does not change, the sample serves as a base.

To learn more about alkali use link below:

https://brainly.com/question/28745372

#SPJ4

Answer:

26 protons..........

The ability of a substance to dissolve in a given solvent at a particular temperature is referred to as solubility.

A compound's solubility refers to its capacity to dissolve in a specific solvent at a particular temperature. Finding how temperature variations affect the compound's solubility properties is a challenge when researching the impact of temperature on solubility.

The issue mostly concerns identifying whether an increase in temperature would lead to an increase in solubility or a decrease in solubility, respectively. Different chemicals can behave very differently. As the temperature rises, some chemicals become more or less soluble, while others become less or less soluble. Additionally, some substances may show just minor modifications or even solubility behavior that deviates from the norm.

Learn more on effect of temperature on the solubility here: https://brainly.com/question/4353941

#SPJ4

Answer:

Pistil connects the stigma to the ovary

There are two properties that can be used to identify an element: the atomic number or the number of protons in an atom. The number of neutrons and number of electrons are frequently equal to the number of protons, but can vary depending on the atom in question.

Answer:Liquids expand for the same reason, but because the bonds between separate molecules are usually less tight they expand more than solids.

Explanation:Heat causes the molecules to move faster, (heat energy is converted to kinetic energy ) which means that the volume of a gas increases more than the volume of a solid or liquid.

Answer:

don't know just supporting u

Answer:

I found this, hope it works

Explanation:

As the mass of the noble elements increases the boiling point gradually decreases and the graph of the two parameters is a straight line graph with downward slope.

It is defined as a substance which cannot be broken down further into any other substance. Each element is made up of its own type of atom. Due to this reason all elements are different from one another.

Elements can be classified as metals and non-metals. Metals are shiny and conduct electricity and are all solids at room temperature except mercury. Non-metals do not conduct electricity and are mostly gases at room temperature except carbon and sulfur.

The number of protons in the nucleus is the defining property of an element and is related to the atomic number.All atoms with same atomic number are atoms of same element.

Learn more about element,here:

https://brainly.com/question/24407115

#SPJ2