There is a healthy bacterial culture growing in the exponential phase . A technician wants to make a growth curve by making cell counts over the day . She plans to take the samples every 30 minutes during the day , hold the samples at room temperature , and then process all samples together at the end of the day .
A) what is the problem with her plan?
B)can she do anything to improve the accuracy of her results ?
Answers
A) WHAT IS THE PROBLEM WITH HER PLAN?
Binary fission is one of the asexual process by which most bacteria reproduce, the binary fission brings about a doubling of the number in bacterial cells that are viable.
Generation time is that time that it will require for a cell population to divide and double, it is also known as doubling time. This Generation time varies among distinct organisms and also varies among culture conditions.
Take for instance,a fast growing bacterium that is under an ideal culture conditions has a doubling time of 20minutes and for the bacteria that is grown in a condition that is not so ideal,may have a doubling time of hours.
The major challenge/ problem with her plan is that as she takes the bacterial sample every 30minutes, and then hold the sample till the last sampling is done in the day,those bacterial cells that are collected in the initial intervals will then get divided and further gives a result that is false.
However,if those bacteria that are collected aren't stored in a culture environment that is standard,the bacterial cells may die off and thus will further bring about a false result.
2) CAN SHE DO ANYTHING TO IMPROVE THE ACCURACY OF HER RESULT?
Solution for rectifying the error in her plan;
She can go ahead and add 50-100 μl of formaldehyde to all the culture suspensions that she has and has taken every 30minutes.
As the formaldehyde will fix the bacterial cells,The optical density present in all those aliquots can then be retrieved/taken at the end of the day.
Looking at it alternatively, aliquot 1ml of the culture suspension at an interval of every 30 minutes,and then take the optical density that is OD at a wavelength of 600nm making use of spectrophotometer until the reason you get becomes static.
Related Questions
As the warm air rises through the Troposphere it:
a. contracts and cools
b. expands and cools
c. expands and heats
d. contracts and heats
Answers
Answer:
B
Explanation:
expands and cools water vapor condenses and clouds develop
select all the body and cellular processes that require cell division (mitosis or meiosis) to function- growth and development- reproduction- replace dead cells
Answers
Both meiosis or mitosis result in cell division to produce new cells. So, for sexually reproducing creatures to continue to exist, these two processes are necessary. The non-sex cells reproduce during mitosis.
Are mitosis and meiosis crucial for development and repair?The form of cell division essential for body growth and repair is called mitosis. Cell division known as meiosis is what creates gametes, or sex cells, for the body. All somatic cells, or cells other than gametes, undergo mitosis throughout the body.
What is the name of the cell division process known as mitosis?Chromosome alignment and segregation are processes that go through distinct phases. After mitosis, a process known as cytokinesis divides the entire cell in half (Figure 1). Walther Flemming's illustration of chromosomes in mitosis, made around 1880, is shown in Figure 1.
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Anyone able to help out with this? :)
Answers
Answer:
A
Explanation:
Look at the graph at 1000, and then look at it again at 2000, then subtract the amount from year 2000 from 1000, and the answer is 135, and you can see at the graph, it goes up, meaning it would be an increase of 135 ppm
Can I have brainliest? It would help me out, if not thanks anyways! Hope this helped and have a nice day :)
EachlinerepresentsYearisaboutppmandyearisaboutppm.Thisgivesusppm.A=AnIncreasrefertothegraph)ifppm.P.s.Thist
The interior of the phospholipid bilayer is _______ meaning it repels polar molecules, such as water
Answers
The cell membrane's inside is hydrophobic, meaning that water won't cling to it. In order to effectively separate fluid inside the cell from fluid outside the cell, phospholipids form a two-layer cell membrane.
Why do polar molecules repelled by the phospholipid bilayer?Large hydrophilic polar or ionic molecules find it difficult to pass through the phospholipid bilayer. The cell membrane prevents the passage of any size charged atoms or molecules.
What component of the phospholipid bilayer is water-repellent?While the tails (the lipid component) are non-polar, the heads (the phospho part) are polar. The tails, which face the inside of the cell membrane, are "hydrophobic" (fearful of water), whereas the heads, which make up the exterior and inner linings, are "hydrophilic" (lovers of water).
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Distinguish between herbaceous and woody stems
Answers
Answer: herbaceous plants are plants with very flexible stems. Their leaves and stems die down to soil level at the end of every growing season amd woody plants are plants with very strong and not easily bendable stems such as trees. Woody plants produce wood as a structural tissue
What proportion of purple kernels is expected in the progeny of the monohybrid cross?
Answers
......................................................
Amanda hates her new student ID photo. Her hair looks dry, the extra weight she's gained shows, and her neck looks fat. Amanda's also been feeling very tired and mentally dull. Amanda goes to the clinic where you are working. You draw her blood. The test results show her T4 levels are low and her TSH levels are low. Later she is given a TSH stimulation test in which TSH is injected and the T4 levels are monitored. After TSH injection her 14 levels rise. A) Does Amanda have problems with her pituitary or with her thyroid gland? B) How did you come to your conclusion?
Answers
Answer:
A) Pituitary Gland B)By analysing the given observations in the question.
Explanation:
THS, thyroid stimulating hormone, is an hormone produced by the pituitary gland and in return it stimulates the thyroid gland that produces hormones such as T3 and T4.
We can conclude from the given information that Amanda's thyroid gland does not have a problem with producing and secreting the T3 and T4 hormones when THS is present in her body.
But the low THS levels show that the problem is with her pituitary gland causing her to have low levels of THS.
We can reach this decision by analysing the given information.
I hope this answer helps.
Explain five ways HIV can change your life
Answers
Answer:
It can change the way your peers and people view you as an individual
Effect the way you view yourself
Can cause a broken relationship
thats it
Explanation:
Diffusion in membrane cell
Answers
Got this off google but still hope it helps :)
Answer:
when small molecules pass through the lipid bilayer of a cell membrane simple passive diffusion occurs
Explanation:
Diffusion is one form of passive transport that doesn't require the expenditure of cellular energy. A molecule can diffuse passively through the cell membrane if it's lipid-soluble, uncharged, and very small, or if a carrier molecule can assist it. ... The assisted process is known as facilitated diffusion.
Which of the following kingdom includes algae?
Answers
Answer:
kingdom prpstimA
Explanation:
i believe
Hot summers and cold winters, topsoil rich in organic material, annual precipitation of 75 to 125 cm (30-50 in), and many hardwood trees are characteristics of the:
Answers
Complete question:
Hot summers and cold winters, topsoil rich in organic material, annual precipitation of 75 to 125 cm (30-50 in), and many hardwood trees are characteristics of the:
A. temperate deciduous forest.
B. temperate grassland.
C. temperate rain forest.
D. boreal forest.
E. chaparral.
Answer:
A. temperate deciduous forest
Explanation:
Temperate deciduous forest
Temperate deciduous forest is one of the biomes that extends to the south of the boreal forest, expanding in regions where temperatures are not that cold, and precipitations are abundant. The temperate forest is not continuous, it extends between 30º and 60º latitude in both hemispheres, and along with the continental extremes, influenced by the oceans. Vegetation suffers changes through the four seasons, which are clearly differentiated. Temperate forest characterizes for having deciduous trees among other phanerophyte species. Due to environmental conditions, these plants need to lose their leaves every year during the cold and dry autumns and winters, as it is the most limiting period over the year. They need to store different nutrients that will be used for plant growth during the following spring. Foliage reappears again during spring and summer.
Winters are cold and summers are warm. Precipitations are abundant, between 750 and 1500 annual mm, and they are well distributed during the year. Usually, there is a small water deficit at the end of the summer, while during the spring there is water excedent in the ground. Soils are very fertile and deep, due to the leaves´ loss that provides nutrients to the soil and protects it from erosion.
Which two environmental changes would be likely to make an ecosystem less
stable?
A. A wildfire rages through a large portion of a forest.
B. A relatively wet summer leads to a larger plant population in a
desert.
C. A volcanic eruption wipes out organisms on an island.
D. A dry spring results in fewer seeds and seedlings on a prairie.
Answers
(Option A) A wildfire rages through a large portion of a forest would be likely to make an ecosystem less stable.
what is an environmental?The term "natural environment" or "natural world" refers to all naturally occurring, that is, unmanmade, living and non-living phenomena. The phrase is most frequently used to refer to the Earth or specific regions of the Earth.
what is an environmental changes?Environmental change is the alteration or disturbance of the natural environment, most frequently brought on by human activities and ecological processes. Various variables, such as natural disasters, human meddling, or animal contact, might cause changes in the environment.
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What day does summer begin in the southern hemisphere
answer options:
A. June 21
B. March 21
C. December 21
D. September 21
Answers
Answer:
The answer is C December 21
Explanation:
Answer:
June 21 trust me i got it right on edge
Explanation:
Which organiam would survive an acidic environment
Answers
Answer:
acidophiles
Explanation:
Acidophiles are organisms that can withstand and even thrive in acidic environments where the pH values range from 1 to 5.
Decide if the function is an exponential growth function or exponential decay function, and describe its end behavior using limits.
Answers
Function is an exponential growth function or exponential decay function it is an exponential decay function, as it starts at infinity and ends at 0.
For exponential functions, we see that our end behavior goes to infinity as our input values get larger. The larger the base of our exponential function, the faster the growth. For logarithmic functions, our function grows slowly as our input values get larger.
Examples of such phenomena include the studies of populations, bacteria, the AIDS virus, radioactive substances, electricity, temperatures and credit payments, to mention a few. Any quantity that grows or decays by a fixed percent at regular intervals is said to possess exponential growth or exponential decay.
Based on the shape of the exponential decay graph, we have the following end behavior: As , x → − ∞ , . y → ∞ . As , x → ∞.
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When ants get food from plants and protect the plants from being eaten, what kind of relationship do these two species have? Plzzzz help!!!
a) mutualistic
b) parasitic
c) commensalistic
d) harmful
Answers
Answer:
Myrmecophytes, otherwise known as ant-plants, share a mutualistic relationship with a colony of ants. This is when two organisms of different species each benefit from the activity of the other. There are over 100 different genera of myrmecophytes that can be found across the world's tropical regions
Explanation:
Define earthquake (dont goo gle)
Answers
Answer:an earthquake
Explanation:an earthquake is a violent shaking of the ground and it also creates greate destruction
B. Exercises
Exercise 1 True or False
Directions: Write Tif the statement is TRUE and Fif the statement is FALSE in your
answer
sheet.
1. T The installation of pipe is plumbing.
Steel piping is designed for a long list of applications.
3. Cutting Tools used in measuring number relating the item under study and the referenced
unit of measurement.
Measuring tools help hand tools and power tools work together, especially important for the
variety of drilling tasks that get done in woodworking.
5. Holding Tools used for holding firmly any material that has to be cut.
6. Indicates the quantity and unit of materials still available in the stockroom is the Balance on
Hand.
7. Unit writes the unit of measuring quantity.
8. A price of material per piece or per unit is Unit Price.
9. The one who signed a signature in a borrower's slip is a borrower.
10. Job Order is not workings sheet that server as a complete guide of the student to a certain
task to be done.
11. Materials and tools are not in accordance with specifications.
12. Check the tools with accessories. See to it that nothing is missing.
13. Check if the materials and tools are of correct specifications,
14. Get replacements for those unreturned materials or tools.
15. Take the issued materials and tools to the workplace.
Answers
Answer:
True
Explanation:
exercise can out use food is bad thing in body work smell feeling bad out anut
A flower has two alleles for petal color "R" for red and "Y" for yellow. Flowers with the genotype RR are red. Flowers with the genotype YY have yellow flowers. And flowers that have the genotype RY have orange flowers. What does that mean about the R and Y alleles?
Y is dominant
R is recessive
R is dominant
R and Y are incompletely-dominant
Answers
Explanation: RY leads to an orange flower, which is a blend of R (red) and Y (yellow). Blends of phenotypes occur in incomplete dominance.
How does mitochondrial structure contribute to aerobic metabolism, particularly in the integration of the citric acid cycle and electron transport?.
Answers
The reduced electron carriers generated by the citric acid cycle are restricted to the matrix by mitochondrial structure. There, they are adjacent to the respiratory complexes of the electron transport chain, which will transfer the electrons from the carriers made by the TCA cycle to oxygen, the final recipient of electrons and hydrogens.
What is the role of mitochondria in the citric acid cycle and electron transport chain?
Citric Acid Cycle:
The pyruvate molecules generated at the conclusion of glycolysis are carried into mitochondria, which are locations of cellular respiration, in eukaryotic cells. By eliminating a carbon dioxide molecule in the mitochondria, pyruvate is converted into a two-carbon acetyl group, which is then taken up by the carrier substance coenzyme A. (CoA). The end product is known as acetyl CoA.
The citric acid cycle occurs in the matrix of the mitochondria in eukaryotic cells. The eight phases of the cycle are a sequence of chemical processes that result in the production of two molecules of carbon dioxide, one molecule of ATP, and the reduced versions of NAD+ and FAD+, known as NADH and FADH2, which are crucial coenzymes for cell function. Because the NADH and FADH2 generated must transport their electrons to the system's next pathway, which uses oxygen, part of this is regarded as an aerobic pathway. This transmission is not possible without oxygen.
Electron Transport Chain:
However, the majority of the ATP produced during the aerobic breakdown of glucose does not come from these mechanisms. Instead, it results from a procedure that starts with the transfer of electrons through a series of chemical events to an ultimate electron acceptor, oxygen. Both eukaryotic organisms' mitochondrial inner membranes and prokaryotic organisms' inner cell membranes contain specific protein complexes where these processes occur. A gradient of electrochemistry is produced across the inner mitochondrial membrane by harnessing the energy of the electrons. ATP is produced using the potential energy of this gradient. This entire procedure is known as oxidative phosphorylation.
The sole aspect of metabolism that employs ambient oxygen is the electron transport chain, which is the final stage of aerobic respiration. The electron transport chain is made up of the aggregation of the four protein complexes I through IV as well as any related mobile, accessory electron carriers.
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What did Elodea, Muscle, Fungus all have in common?
They are made of cells
O They shared the same magnification amount
They were the same size
Answers
Answer:
The correct option is They are made of cells
Explanation:
Cell is the structural and functional unit of life. Although, the three specimen given are of different "classes" but they are all of living origin. And all living things are made up of cells. For instance, Elodea is an aquatic plant (popularly called waterweed) is made up of plant cells. While Muscle is a tissue found in many animals and is made up of cells (muscle cells/myocytes); since cells generally join together to form tissues. Fungus is a eukaryotic organism and hence is also made of cells.
What is a circulatory ?
A. heart blood vessels and blood
B.Skin hair nails
C. Bones
D. Brain spinal cord and nerves
E. Lungs nose pharynx and trachea
F. Mouth stomach esophagus small and large intestine anus
Answers
heart bloodvesselsand blood
A crime scene has the following blood splatter against a wall 2 feet from where the victim was standing what type of impact would have caused this pattern 
Answers
Answer:How It’s Done. Bloodstain Patterns that May be Found. Bloodstains range in both amount of blood and type of pattern—from pools of blood around a body to obvious spatter patterns on the walls to microscopic drops on a suspect’s clothing.
Explanation:
You have been tasked with writing a blog for a popular website that discusses controversial topics. The topic you have been given is GMOs, or genetically modified organisms. Make sure to answer the following questions in your blog:
What are GMOs and give 2 examples.
List 2 benefits and 2 risks of using GMOs.
State your position (are you for or against the use of GMOs) regarding GMOs.
Provide at least 2 reasons for your position.
Answers
The main goals of GM crops typically include resistance to specific pests, diseases, or environmental factors as well as resistance to chemical treatments.
Why are genetically modified organisms?An organism that has had its DNA altered through the use of genetic engineering methods is referred to as a genetically modified organism (GMO). Breeding techniques have been used by humans to alter creatures for thousands of years. Dogs, livestock, and even corn have all undergone years of selective breeding to develop specific desired features.
The common goals of GM crops include resistance to specific pests, diseases, or environmental factors as well as resistance to chemical treatments (e.g. resistance to a herbicide). Enhancing a crop's nutritional value is another reason for genetic manipulation, as is the case with golden rice.
DNA insertion into an organism's genome is a technique used in genetic engineering (GE).
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As a result of an action potential, AcH is released from the axon terminal and attaches to receptors on the motor end plate. Sodium rushes in the T-tubules and all the steps occur until the muscle contracts in the leg and carry out the command transported by the motor neuron. Which gray horn is the cell body of the motor neuron located? Infer and explain if this is a positive or negative feedback
Answers
1) The cell body of the motor neuron is located in the ventral or anterior horn of the grey matter. From there, it extends to the motor unit. 2) Contraction refers to negative feedback in which the body reacts when facing a stimulus.
What is the anatomy of the spinal cord?The spinal cord is mainly composed of grey matter and white matter. The second one covers the first one.
The grey matter transversal section is composed of posterior, lateral, and anterior horns, also known as dorsal, lateral, and ventral horns, respectively.
Each type of horn contains a type of neuron.
In the posterior/dorsal horns, we can find sensory neurons that provide sensory information.In the thoracic area, we can find lateral horns with visceral motor neurons.
In the anterior/ventral horns, we can find motor neurons that extend to the skeletal muscle, which is the motor unit, to produce movement.
The motor neuron from the exposed example is located in the anterior or ventral gray horn.
This example refers to negative feedback because the neurotransmitter release causes a cascade effect that ends in muscle contraction, tending to suppress or counter the original stimuli.
Any change that might occur in the internal functioning will be countered by negative feedback to maintain homeostasis.
Negative feedback needs receptors, central control, and effectors.
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Please Help
Sex-Linked Disorder
Answers
Explanation:
hemopholia
colour blindness
Compare and contrast the characteristics of the ideal tissue forming the outer surface of the body with that forming the lining of the small intestine. How are they similar and different? What tissues fulfill these needs?
Answers
The tissue that forms the outer surface of the body is a stratified squamous keratinized epithelium. The surface layer of dead cells(stratum corneum) is made to keep things out of the body, protecting it. The tissue that lines the digestive tract(e.g. intestine). Is made from 1 layer of columnar cells that facilitate the absorption of nutrients. Both tissues are epithelia.
The tissue that forms the outer surface of the body is a stratified squamous keratinized epithelium.
It should be noted that the cells on the surface of stratified squamous keratinized epithelium are very flat. The surface layer of dead cells is vital for protecting the body.
The tissue that lines the digestive tract that is, the intestine is made from a layer of columnar cells that is important for the facilitation of the absorption of nutrients.
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which of the following most closely resembles cellular respiration
Answers
Logs burning in a campfire are the circumstances that most mimic cellular respiration. This is due to the fact that both processes release energy and depend on oxygen to continue. The body uses cellular respiration, a series of metabolic events, to liberate energy from food.
what is respiration?This is the process of breathing; the taking in and exhaling of air. Biology. the whole of the physical and chemical mechanisms that transport oxygen to tissues and cells and release carbon dioxide and water during oxidation in an organism.
what does metabolic events?It is absolutely necessary for an organism to preserve its life for a process known as metabolism to take place in its body. These activities, which include anabolism and catabolism, are also known as metabolic processes.
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why life process lead to an increase in the number of organism
Answers
Answer:
my life kinda sucks maybe
Explanation:
but i have my nice family sry for not answer i have no brain so peace out
Depressants are drugs that?
Answers
Answer:
Depressants are drugs that slow down the central nervous system, leading to reduced brain activity, decreased heart rate, and relaxed muscles.
Explanation: