the disc diffusion method should be read out in 24 hours after inoculation. what errors can occur allowing plates to sit longer than 24 hours?

Answers

Answer 1

Errors such as distorted areas, and overgrowth can occur during the disc diffusion method.

What errors can occur allowing plates to sit longer than 24 hours?

If the plates are left longer than 24 hours, a few errors can occur, which are discussed below:

Distorted zones: When a plate is left longer than 24 hours, the inhibition zones that were observed in the initial reading might become distorted, making it difficult to measure the correct diameter of the zone, which can lead to inaccurate results.

Overgrowth: After 24 hours, some organisms, such as Pseudomonas aeruginosa, can overgrow and can lead to a false-negative result.

Merging of zones: If there are multiple antibiotic disks, merging of zones may occur when left for a longer period than 24 hours. This is because, after 24 hours, some antimicrobial agents are not stable and can diffuse from the disk into the surrounding media, leading to the merging of the zones.

In conclusion, if plates are left longer than 24 hours, the inhibition zones can become distorted, the overgrowth of some organisms can occur, and the merging of zones can occur if multiple antibiotic disks are present.

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Related Questions

the ""neural signature"" of dyslexia seems to be impaired processing in the brain’s...

Answers

The neural signature of dyslexia appears to involve impaired processing in the brain's left hemisphere regions responsible for reading and language comprehension.

This includes the inferior frontal gyrus, the superior temporal gyrus, and the angular gyrus.

These regions are important for decoding and integrating phonological and semantic information.
An explanation for this impaired processing is that individuals with dyslexia may have differences in brain structure and connectivity, as well as decreased activation in these regions during reading tasks.

Additionally, dyslexia may be influenced by genetic and environmental factors, such as prenatal exposure to stress or toxins.


In summary, the neural signature of dyslexia involves impaired processing in the left hemisphere regions responsible for reading and language comprehension, potentially due to differences in brain structure and connectivity, decreased activation during reading tasks, and genetic and environmental factors.

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How can proteins interact with dna and recognize dna sequences without unwinding the dna double helix? multiple choice question

Answers

Proteins can interact with DNA and recognize DNA sequences without unwinding the DNA double helix. How.Proteins can interact with DNA and recognize DNA sequences without unwinding the DNA double helix because of their specific DNA-binding domains. Proteins that interact with DNA can include transcription factors, DNA helicases, and DNA polymerases.

These proteins are capable of recognizing specific DNA sequences by using their specific DNA-binding domains, which can include zinc fingers, leucine zippers, helix-loop-helix, and homeodomains. These domains can interact with the DNA helix and enable proteins to recognize specific DNA sequences without unwinding the DNA double helix.

However, certain proteins can require DNA unwinding to interact with the DNA, such as DNA polymerases that use unwound DNA as a template to replicate DNA sequences during DNA replication. The mechanism that proteins use to interact with DNA sequences without unwinding the DNA.

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according to the information presented in the passage, how would the absorbance at 570 nm change over time for enzyme a if a linear starch were used?

Answers

The absorbance at 570 nm change for enzyme A if a linear starch was used is It would remain relatively constant, option A.

Proteins called enzymes aid in accelerating our bodies' chemical processes, or metabolism. Some compounds are created, while others are broken down. Enzymes are a part of all living things. Enzymes are created by our bodies spontaneously.

Enzyme A probably breaks down 1,6 links, resulting in an increase in linear polysaccharides and absorbance.

The 1,6 non-linear linkages wouldn't need to be broken down, so iodine wouldn't bind more or less to the linear chain and wouldn't affect the absorbance if the starch molecule were linear from the start. If this were the case, enzyme A wouldn't be required to break down those linkages.

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Complete question:

According to the information presented in the passage, how would the absorbance at 570 nm change for enzyme A if a linear starch was used?

A. It would remain relatively constant.

B. It would decrease

C. It would increase

D. It would initially increase and then decrease as the starch is consumed

the main tube that carries air to and from the lungs is called ?

Answers

Answer:

trachea

Explanation:

Answer: trachea

Explanation:

The trachea is an essential tube connecting the larynx to the bronchi of the lungs and allowing air to be carried to and from the lungs. It is commonly known as the windpipe and about four inches long and less than an inch in diameter.

If the independent variable DOES effect the outcome of the experiment, the Chi-Squared value will be ______ the critical value because you are ________ the null hypothesis.

If the independent variable DOES effect the outcome of the experiment, the Chi-Squared value will be

Answers

If the independent variable does affect the outcome of the experiment, the Chi-square value will be greater than the critical value because you are rejecting the null hypothesis.

What is Chi-square?

The Chi-square is a statistical tool used to test the significant difference between two sets of similar data. It is an inferential statistical tool that can be used to test the acceptability or otherwise of null hypotheses.

The Chi-square value from data must be greater than the critical value in order for the null hypothesis of the experiment that serves as the source of the data to be rejected. The conclusion would be that there is no significant difference between the two sets of data.

On the other hand, when the critical Chi-square value is greater than the calculated value, the null hypothesis is accepted and the conclusion would be that there is no difference between the two sets of data.

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the sequence of the coding strand of a dna molecule (that is, the dna strand that contains the codons specifying the protein sequence) is 5'-cggatgctta-3'. what is the sequence of the rna made from this dna?

Answers

Transcription of an RNA strand with the sequence "AUUGCGCGAA" from a DNA strand with the sequence "TAACGCCTT" As a result, C is the correct answer.

Transcription is the process by which mRNA is created from DNA in the nucleus of a cell. The nucleus contains all of the enzymes and nucleotides required for the production of mRNA. The messenger RNA (mRNA) is transcribed from DNA The mRNA sequence generated in a cell from DNA is complementary to the leading strand of DNA, with only the thymine residues replaced by uracil residues. Adenine base couples with uracil residues, and thymine base pairs with adenine residues. Cytosine bases couple with guanine bases and vice versa.

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4) A student wanted to determine if the results for wild-caught spiders tested shortly after they were captured would be the same for
different species of prey. Which of the following experiments should the student conduct?
OF. Repeat Experiment 1 with A. domesticus as the species of prey.
G. Repeat Experiment 3 with A. domesticus as the species of prey.
H. Repeat Experiment 1 with a species of prey other than A. domesticus.
J. Repeat Experiments 3 with a species of prey other than A. domesticus.

Answers

Answer:

Answer would be G

Explanation:

There are two DNA strands in the picture of question 1. These strands run in opposite directions from 5' to 3' we call these.... ​

Answers

Answer: Antiparallel

Explanation:

DNA is composed of two strands of nucleotides. The strands each run from 5' to 3' and run in antiparallel, or opposite, directions from one another.

What role do decomposers play in the food chain?

Answers

Answer: Decomposers breaks down dead or decaying organisms. Decomposers carry out the natural nutrients to decomposition
They break down dead organisms

What 7 traits did Mendel study in peas?

Answers

Seven traits that Gregor Mendel studied in peas include:

pea shape (wrinkled or round),pea color (yellow or green),flower color (white or purple),pod shape (inflated or constricted),pod color (yellow or green),position of flowers (terminal or axial), andplant size (dwarf or tall).

Gregor Mendel (20 July 1822–6 January 1884) was an Austrian biologist that was widely known for his pea plant experiments. Mendel is often called the father of genetics.

He studied the inheritance of seven different traits in peas, which included height, flower and seed colors, and seed shape. To do so, Mendel first established pea lines with two different forms of a trait, such as yellow vs. green color. For generations, Mendel grew these lines until they were pure-breeding (which meant they always produced offspring identical to the parent), and then he bred them to each other and observed how the traits were inherited.

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help...
The graph shows projected changes in the populations of the world. Based on the information in the graph, wich region is expected to have the least population growth over the period shown.
Europe
Asia
Africa
Australia

help...The graph shows projected changes in the populations of the world. Based on the information in

Answers

Based on the graph the region expected to have the least population growth will be Australia.

Graphical illustrations

Looking at the given graph the population size of each region starts at a lower point and ends at a higher point on the graph between the years 1950 and 2050.

The change in the population also varies depending on the scale on the y-axis. Australia has the lowest scale which lies between somewhere above 10 and 50.

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Help plsss I very confused

Help plsss I very confused

Answers

Answer:

isn't that a mitochondria

Explanation:

I would say to provide cells with energy for their activities

Explain the importance of neem, turmeric and blue green algae in agriculture

Answers

Answer:

Blue green algae are used to prepare bio fertilizers  .Neem leaves are used in grain storage as bio pesticides . Turmeric is used in grain storage as bio pesticides  .

Hope this helps.....

Pls mark my ans as brainliest

If u mark my ans as brainliest u will get 3 extra points

Answer:

The answer is

Explanation:

Blue green algae is used in farming.

Why do atoms form bonds with each other

Answers

Answer:

Atoms form chemical bonds to make their outer electron shells more stable. ... An ionic bond, where one atom essentially donates an electron to another, forms when one atom becomes stable by losing its outer electrons and the other atoms become stable (usually by filling its valence shell) by gaining the electrons.

Explanation:

List in order what an athlete does when she bl00d d0pes.

1. She chills the bl00d and stores it.

2. She puts the bl00d back into her body just before a competition.

3. She removes the bl00d from her body.​

Answers

i know nothing on this topic, however the most logical answer would be 3,1,2
pretty easy if you think about it3, 2, 1

What forms as part of mitosis?
A. Four fused gametes
B. Two nuclei with identical chromosomes
C. One complete zygote
D. Three sets of chromosomes​

Answers

Answer:

the answer is B

Explanation:

B is the answer

Answer:

It's B hope this helps!!!

Explanation:

A P E X

The beaks of finches found on various Galápagos Islands differed based on ?

Answers

Answer:

the food that they ate

Explanation:

The beaks of finches found on various Galápagos Islands differed based on the food that they ate. The finches that survived on eating different larger nuts had strong beaks for breaking the nuts open. While the Finches that ate smaller type nuts and seeds had beaks for cracking nuts and seeds. This also applied to other types of animals as well, each adapting to its own necessities.

Question 11 (2 points) The pH of water is 7. A student adds 5 drops of a base to the water changing the pH to 10. The student repeats the experiment but this time, he adds the base to a buffered solution. The pH of the buffered solution does not change (it is 7 before the base is added and it is still 7 after 5 drops of the base is added). The buffer solution is said to "resist" a change in pH. Compare the percentage changes for the water solution and the buffer solution. Do these percentages support a resistance to change for the buffer solution? In your own words, explain the chemical basis of how the buffer resists pH changes when the base is added Explain your answer 5 9 12 15

Answers

The percentage change for water solution is (10 - 7) / 7 * 100 = 42.9%. The percentage change for the buffer solution is (7 - 7) / 7 * 100 = 0%.

These percentages support the resistance to pH change for the buffer solution. Buffers are made up of a weak acid and its conjugate base (or a weak base and its conjugate acid) and are designed to maintain a relatively stable pH. When a base is added to a buffered solution, the buffer components react with the added base, either by accepting protons or by reacting with hydroxide ions, in order to minimize the change in pH. The weak acid component of the buffer can release protons to counteract the base, while the conjugate base component can react with the hydroxide ions. This ability to counteract changes in pH is what allows the buffer solution to resist significant shifts in its pH value. In this case, the buffer successfully resisted a change in pH when the base was added, as evidenced by the 0% percentage change.

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A farmer using conservation blank
methods to cultivate the fields will limit soil erosion and return organic
material and nutrients to the soil, and will reduce soil compaction from repeated tilling, while increasing the chances of
carrying diseases and pests over from prior crops.

Answers

When water or wind impact separates and removes soil particles, the soil deteriorates gradually as a result of soil erosion.

Thus, Around the world, surface runoff, soil erosion, and poor water quality have become major issues. The issue could get so bad that the land would have to be abandoned since it was unusable for farming.

The history of such civilizations serves as a helpful reminder to safeguard our natural resources because many agricultural civilizations have declined as a result of poor land and resource management.

Erosion is a significant issue for both the quality of the water and viable agricultural land. Any soil management strategy that aims to improve the quality of the soil and water must include sediment control as a core component.

Thus, When water or wind impact separates and removes soil particles, the soil deteriorates gradually as a result of soil erosion.

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60 minutes remaining
Question 13 The most abundant photoreceptors that detect dim light are Cones.
A True
B False
Question 14 Muscular tissue that adjusts the shape of the pupil to regulate how much light enters the eye is IRIS.
A True
B False Question
15 Opsins are visual pigments derived from Vitamin D.
A True
B False

Answers

Answer:

Question 13:

B. False

The most abundant photoreceptors that detect dim light are Rods, not Cones. Rods are highly sensitive to low light conditions and are responsible for vision in dim light and peripheral vision. Cones, on the other hand, are responsible for color vision and high visual acuity but are less sensitive to low light conditions.

Question 14:

A. True

The iris is the muscular tissue in the eye that adjusts the size of the pupil, controlling the amount of light entering the eye. It contracts or expands to regulate the size of the pupil in response to changing light conditions.

Question 15:

B. False

Opsins are visual pigments found in photoreceptor cells, specifically in the retina of the eye. They are responsible for capturing light and initiating the process of vision. Opsins are not derived from Vitamin D. Vitamin D is a separate compound involved in various physiological processes in the body, including calcium absorption and bone health.

Explanation:

Give five example molecules​

Answers

These are the five examples for the molecules with the clear definition.

Give five example molecules

During DNA replication each strand of dna is used as a template to produce a complementary strand of DNA This process is shown below which base will attach to location 2

A) Allimene
O
B) Adenine
O
C) Thymine
O
D) Tyrosine

During DNA replication each strand of dna is used as a template to produce a complementary strand of

Answers

I think the answer might be adenine?


Students from a high school science class visited the desert of Southern California and investigated a sample of
sedimentary rock from the region.
Which question can they answer through scientific
investigation?
O Which fossils are found in different layers of sedimentary rock?
Why are sedimentary rocks prettier than other kinds of rocks?
Which layers of a sedimentary rock have the most interesting fossils?
- Why should scientists study sedimentary rocks?

Answers

Answer:

Which fossils are found in different layers of sedimentary rock?

Explanation:

Thats the answer i hope it helped! :)

Answer:

Which fossils are found in different layers of sedimentary rock?

Explanation:

Which fossils are found in different layers of sedimentary rock?

Enzymes retain their shape in extreme temperatures and pH levels.
True or
False

Answers

I believe the answer is True!

8. blocking ribosomes prevents? a. protein packaging b. protein folding c. protein secretion d. protein assembly e. protein absorption

Answers

Blocking ribosomes prevents protein assembly. The correct answer to the question is option d) protein assembly.

Blocking ribosomes can prevent protein assembly, which is the process of assembling amino acids to form proteins. Ribosomes are the primary site of protein synthesis in a cell, and they play a crucial role in the formation of peptide bonds between amino acids. By blocking ribosomes, the formation of peptide bonds is impeded, which can lead to the inhibition of protein assembly.

This can occur due to several factors such as the presence of an antibiotic that prevents the functioning of ribosomes, or the genetic modification of the ribosome, which changes the way it interacts with amino acids. Blocking ribosomes can have significant consequences on the cell's ability to carry out its normal functions, as proteins are essential for the proper functioning of all living organisms.

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In the first experiment, Dr. Palumbi applied heat stress to corals from different pools, warm and cool. Make a
claim regarding the resistance to bleaching of the warm pool corals and the cool pool corals. Justify your
claim with experimental evidence.

Answers

Answer and Explanation:

Corals from the warmer pool (20%) were more resistant to bleaching with a 35% difference to the cooler pool (55%). the resistance to bleaching of the warm pool corals.

The size of the produced amoeba differ by changing the surrounding environment , but its chromosomal number and type of division stay the same by changing the surrounding environment

-The two statements are right
-The two statements are wrong
-The first statement is right and the second is wrong
-The first statement is wrong and the second is right

Answers

Answer:

The first statement is wrong and the second is right

If the codon is GUC, what is the anticodon

Answers

guanine binds with cytosine (C)

uracil binds with adenine (A)

cytosine binds with guanine (G)

so the anticodon in CAG

Answer:

CAG

Explanation:

2. What is the role of wax impressions, and/or plaster casts, of teeth in forensic odontology

Answers

Answer:

to help mold or move the teeth

Explanation:

when you put a piece of wax on a pencil it miles to what the pencil shape is so the impression is either a vaneer or just mold of your teeth

Which model below shows a prokaryotic cells?

Which model below shows a prokaryotic cells?

Answers

Answer:

Modle two as it is singular, simple with a flagellum

Explanation:

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