In this particular experiment, we used red-labeled DNA to process the sample from _____________ tissue and the green-labeled DNA to process the sample from ___________.
Answers
In the experiment, we used red-labeled DNA to process the sample from the cancerous tissue and green-labeled DNA to process the sample from normal tissue. We used the process of in situ hybridization to visualize the genomic DNA inside the cell using a fluorescent probe.
This technique is useful in visualizing and identifying specific DNA sequences in intact cells and tissues. We used different probes labeled with different fluorescent dyes to target the specific genes of interest. The red and green-labeled probes allowed us to distinguish between the normal and cancerous cells. The probes bind to the complementary DNA sequence and the labeled probes give off light, which can be detected using a fluorescent microscope.
In this way, we could observe the difference between the gene expression in normal and cancerous cells. Overall, the use of fluorescent probes and in situ hybridization is a powerful technique for studying gene expression in cells and tissues.
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Related Questions
which two european powers signed the treaty of tordesillas ?
Answers
On June 7, 1494, the state-run administrations of Spain and Portugal consented to the Arrangement of Tordesillas, which partitioned their authoritative reaches in the "New World" of the Americas.
The Arrangement of Tordesillas, June 7, 1494. On June 7, 1494, Spain and Portugal consented to fix the limit between their individual spaces along a meridian 370 associations west of the Cape Verde islands. They laid out two regions in which they would have an imposing business model over disclosure, route, and exchange.
Be that as it may, John II dissented and started discussions prompting the observed Settlement of Tordesillas (June 1494), which provided for Spain all terrains west of a line 370 associations toward the west of the Cape Verde Islands.
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DNA polymerases also have nuclease activities. Which of the following is not a use for the nuclease activity of DNA polymerase I?
-proofreading during synthesis
-trimming single-stranded ends
-removal of RNA primers
-degradation of viral DNAs
-removal of DNA lesions during repair
Answers
The use of the nuclease activity of DNA polymerase I that is not related to its function is degradation of viral DNAs. DNA polymerases have nuclease activity that helps in proofreading during synthesis, trimming single-stranded ends, removal of RNA primers, and removal of DNA lesions during repair.
DNA polymerase I has both 5' to 3' polymerase and exonuclease activity, which enables it to remove RNA primers and to proofread during DNA synthesis. The 3' to 5' exonuclease activity of DNA polymerase I helps in removing DNA lesions during repair by cutting out the damaged nucleotides from the strand. Additionally, it can also trim single-stranded ends during DNA replication, which is required to create blunt ends for ligation in cloning procedures. However, DNA polymerases do not have a specific nuclease activity for the degradation of viral DNAs. Thus, the nuclease activity of DNA polymerase I is primarily involved in DNA synthesis, repair, and modification and not degradation of viral DNAs.
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Please explain the following 2 questions in the image.
The reasoning should be correct and clear.
PLZ HELP NEEDED ASAP!!!
BRAINLIEST AND XTRA POINTS!!!
NO LINKS!!!!
YOU WILL GET REPORTED!
Answers
1. Carbohydrates
2. Proteins
PLEASE HELP!!! WILL GIVE BRAIN!!!
Answers
Answer:
Community!!!
Explanation:
A population includes only the SAME types of animals.
A biosphere is the region where they live in, so it has abiotic factors
abiotic are NONLIVING THINGS.)
The community, has different animals, and that's it.
Lastly, and ecosystem, is in a biosphere, but includes the community, and abiotic things.
I hope this helped, and I am here if you need anything else!
6. List three economic benefits of biodiversity.
Answers
Explanation:
Provision of wild harvested food products such as fish, large and small animals, and maple syrup. Provision of medicinal plants and raw materials for pharmaceuticals. Enabling nature-based tourism and the hunting and fishing industry.
The two populations are similar, but one is slightly more of a specialist in its diet, whereas the other is more of a generalist. Which population is more of a specialist? O Population B O Population A Read each of the following passages. After each passage, choose the term that most appropriately completes the sentence.
Answers
The population that is slightly more of a specialist in its diet would be Population A.
In ecology, a specialist is an organism that has a narrow diet and can only thrive under specific conditions, while a generalist can survive on a wide variety of food sources and environmental conditions.
The passage suggests that both populations have similar characteristics but one is "slightly more of a specialist in its diet." Therefore, Population A must have a narrower and more specific diet than Population B, making it the specialist population.
It's important to note that the passage does not provide explicit information on the diets of either population, nor does it define what "slightly more of a specialist" means in terms of the percentage of the diet that is specialized.
However, based on the given information, we can infer that Population A is more specialized than Population B, albeit only slightly.
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Complete question:
The two populations are similar, but one is slightly more of a specialist in its diet, whereas the other is more of a generalist. Which population is more of a specialist? Answer the result from graph shown below
O Population B
O Population A
HELPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPP
Explain please
Answers
Answer:
Site A W,X,Y,Z
Site B W,Z,Y,X
Explanation:
this is true believe meCan someone explain how it is possible for a single molecule of steroid hormone to create a significant and lasting response in an organisms cell?
Answers
Answer:
half life and higher affinity
Explanation:
it is because they have long half life in blood and high affinity
Steroid hormone
The cell signaling pathways induced by the steroid hormones regulate specific genes within the cell's DNA. The hormones and receptor complex act as transcription regulators by increasing or decreasing the synthesis of mRNA molecules from specific genes.Steroid hormones are not able to target every cell within the body, so the overall response is slower. They bind to receptors on the cell's surface and the receptors aid in helping the steroid hormones enter the cell.Hormones work by binding to protein receptors either inside target cells or on their plasma membranes. The binding of a steroid hormone forms a hormone-receptor complex that affects gene expression in the nucleus of the target cell.Steroids pass into a cell's nucleus, bind to specific receptors and genes and trigger the cell to make proteins.Learn more:
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Why does friction cause objects to slow down?
Answers
Answer:
Friction is a force that occurs when two surfaces slide past one another. The force of friction opposes the motion of an object, causing moving objects to lose energy and slow down.
Explanation:
Brainlest
How would the bhopal disaster have been handled differently if it took place in the US 
Answers
The 1984 gas leak in Bhopal, India destroyed thousands of lives; but in the US, the disaster led to better regulation of industrial hazards.
What is disaster?
A catastrophe is a sudden, unavoidable event that results in significant harm, devastation, fatalities, or suffering. Disasters can be brought on by human activity or they can occur naturally (such as earthquakes, hurricanes, or wildfires) (such as oil spills, industrial accidents, or terrorist attacks).
A disaster's effects can be felt widely, affecting not only the people who are directly impacted but also their families, communities, and larger society. In the immediate aftermath of a disaster, efforts are concentrated on search and rescue, providing basic necessities like food and shelter, and stabilising the affected area.
Rebuilding infrastructure, assisting communities and businesses in getting back on their feet, and attending to the physical, emotional, and financial needs of those affected may all be part of the long-term recovery process.
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sprouted seeds un sprouted seed taken in a jar with lime water in both in which jar lime water changes it colour fast
Answers
Answer:
Correct option is White
Explanation:
Germinating seeds carry out their
respiration process at the cellular level to
be able to generate energy for growth and
maturation.
They do not yet have the ability to carry out
photosynthesis.
As a result of respiration, carbon dioxide is
released by the seeds which turn lime
water milky or white.
So the correct answer is 'White
Question 7 of 10
What type of active reading strategy are you using when you come up with
pictures in your head that help describe the text?
A. Making predictions
O B. Monitoring and applying fix-up strategies
C. Making mental images
D. Using text features and visual cues
Answers
Answer:
using text features and visual crues
making predictions
Wavelength is 6.25x10^7 m whats the frequency
Answers
Answer:
\(\huge\boxed{\sf f=4.8 \ Hz}\)
Explanation:
Given Data:Wavelength = λ = 6.25 × 10⁷ m
Speed of light = c = 2.998 × 10⁸ m/s
Required:Frequency = f = ?
Formula:\(\displaystyle f=\frac{c}{\lambda}\)
Solution:Put the givens in the formula.
\(\displaystyle f=\frac{2.998 \times 10^8}{6.25 \times 10^7} \\\\f=0.48 \times 10^1\\\\f=4.8 \ Hz\\\\\rule[225]{225}{2}\)
organisms that gain energy by consuming preexisting organic molecules are called:
Answers
Answer:
heterotrophs
Explanation:
A majority of the seed is made up of the...
a) seed coat
b) testa
c) endosperm
d) radicle
Answers
Answer:
Your answer is C) endosperm.
What is the complementary sequence of TACGTATGAAAC?
Answers
Answer:
ATGCATACTTTG
Explanation:
The letters are alternate of each other.
T-A
A-T
G-C
C-G
ATGCATACTTTG is the compementary sequence
Which statements correctly describe the digestive system? Select four options.
Food is cut into pieces in the mouth.
Food becomes dry in the mouth.
Proteins are broken down in the stomach.
Carbohydrates are first broken down in the stomach.
Food becomes mostly solid in the large intestine.
Fat is broken down into smaller droplets in the small intestine.What is peristalsis and where does it occur?
Answers
Answer:
1,3,5,6
Explanation:
The intertidal areas of marine ecosystems and the littoral zone of a lake are both located where water meets land. Do you
think most organisms that live in the littoral zone could also live in an intertidal area? Why or why not?
Answers
Answer:
yes becuase they do have some similar characteristics
Explanation:
The cone-shaped landform shown in the image above is located in northeastern New Mexico. This landform is a _______, and it was created by a _______ process.
A.
fault; destructive
B.
sand dune; destructive
C.
glacier; constructive
D.
volcano; constructive
Answers
Answer:
D. Volcano;constructive
Explanation:
bones do all of the following except - f make nerve cells g store calcium h protect organs j make blood cells
Answers
Bones do all of the following except "make nerve cells." However, bones do play an important role in the body's nervous system by providing protection for the spinal cord and brain. Bones also store calcium, which is essential for muscle and nerve function, and they protect organs such as the heart and lungs.
Additionally, bones produce red and white blood cells, which are necessary for the body's immune system and oxygen transportation.
Bones perform various functions such as storing calcium, protecting organs, and making blood cells.
However, they do not make nerve cells. In summary, bones do all of the following except make nerve cells (option F). Nerve cells, also known as neurons, are primarily formed in the nervous system.
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cancer is a disease in which cells?
A. grow and divide on controllably.
B. die before they can mature
C. start producing DNA.
D. die during Mitosis
Answers
A grow and divide uncontrollably.
Answer:
A
Explanation:
cancer cells divide uncontrollably
Based on the data collected and what you have learned about osmosis and the three treatment options, rank the treatments from most effective to least effective. What do you predict?
Answers
Although I could not locate the options in order to provide a ranking, I can offer the insight that a hypertonic saline solution will be the most effective form of treatment.
I believe you are referring to the full case study which says :
"Your goal is to stop Clark's seizures by restoring equilibrium between the blood and the matrix. Based on the data collected and what you have learned about osmosis and the three treatment options, rank the treatments from most effective to least effective. What do you predict?"
A hypertonic saline solution is used to draw water out of cells by creating an increased concentration of solute in the blood, forcing the water out of the cell through osmosis.
Clark's seizures may be caused by Increased solute concentrations in the brain which forces water from blood to move into the brain, thus increasing the matrix pressure. This then causes the neurons to fire at an increased rate and leads to the seizures Clark was experiencing.
Hypertonic saline can be used to increase solute concentrations, in this case, Sodium, in the blood which forces water to move back into veins. Thus returning Clark to a balanced level of blood and matrix water levels, and may reduce his likelihood of seizures.
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Answer: A hypertonic solution would be most effective. Isotonic Saline would be least effective and so would Hypotonic.
Explanation:
People who have leukemia, a cancer that affects white blood cells, are often given Cytarabine. This drug inhibits the synthesis of DNA. Which phase of the cell cycle is most affected by Cytarabine?
A
Interphase
B
Cytokinesis
C
Prophase
D
Anaphase
Answers
Answer:
I think interphase because fna synthesis happens within the s-phase of the cell cylce and 5he s phase is suring interphase and not mitosis
The phase of the cell cycle which is most affected by Cytarabine is the Interphase. Thus, the correct option is A.
What is the cell cycle?The cell cycle may be defined as the entire sequence of events happening from the end of one nuclear division to the beginning of the next. The entire cell cycle can be divided into two phases.
The InterphaseThe M-phase (division phase).As per the question, the drug Cytarabine inhibits the synthesis of DNA, it definitely inhibits the interphase of the cell cycle. This is because the synthesis of DNA occurs in the S-phase of the cell cycle which is a substage of the interphase.
Therefore, the interphase of the cell cycle must be affected by the drug Cytarabine. Thus, the correct option for this question is A.
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Which is the role of restriction enzymes?
to isolate the selected gene
to cut DNA into fragments of different lengths
to move and separate the strands of DNA
to join the sticky ends of DNA fragments
Answers
Answer:
to cut DNA into fragments of different lengths
Explanation:
A restriction enzyme is a protein that recognizes a specific, short nucleotide sequence and cuts the DNA only at that specific site, which is known as restriction site or target sequence.
More than 400 restriction enzymes have been isolated from the bacteria that manufacture them. In live bacteria, restriction enzymes function to defend the cell against invading viral bacteriophages. Restrictions sites in the viral genome (a "happy accident" of nature, as far as the bacteria are concerned, since they don't appear to have any specific function in the virus) are cleaved by the bacterium's restriction enzymes, fragmenting and destroying the DNA of invading bacteriophages before it can incorporate into the host's genome and take over the cell.
A bacterium is immune to its own restriction enzymes, even if it has the target sequences ordinarily targeted by them. This is because the bacterial restriction sites are highly methylated, making them unrecognizable to the restriction enzyme.
Isn't evolution fantastic?
The correct answer is option C i.e. to cut DNA into fragments of different lengths.
what is restriction enzyme?A restriction enzyme is a bacterial protein that cleaves DNA sequences at sequence-specific places, resulting in DNA fragments with known sequences on both ends.
Certain laboratory processes, such as recombinant DNA technology and genetic engineering, require the use of restriction enzymes.
There are four different types of restriction enzymes: type I, II, III, and IV, which differ principally in structure, cleavage site, specificity, and cofactors.
Both restriction and methylase functions are carried out by a single big enzyme complex in Types I and III enzymes.
The restriction enzyme is independent of its methylase in the Type II system.
Several thousand type II restriction enzymes have been identified from a variety of bacterial species.
These enzymes recognize a few hundred distinct sequences, generally four to eight bases in length.
Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites.
Type IV restriction enzymes cleave only methylated DNA and show weak sequence specificity.
hence, the correct answer is option B.
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15. Explain how the speed of a stream affects the suspended load.
Answers
Answer:
As a stream flows faster, it can carry larger and larger particles.
Explanation:
Unlike dissolved load, the size of the particle that can be carried as suspended load is determined by the velocity of the stream. As a stream flows faster, it can carry larger and larger particles. The larger the size particle that can be carried by a stream, the greater the stream's competence.
Biology - need ASAP. will mark brainliest
Describe the main ways in which the blood helps you to avoid infection. Include a description of the parts of the blood involved.
(6 marks)
Answers
Answer:
Blood brings oxygen and nutrients to all the parts of the body so they can keep working. Blood carries carbon dioxide and other waste materials to the lungs, kidneys, and digestive system to be removed from the body. Blood also fights infections, and carries hormones around the body.
Explanation:
As for Blood AB it’s rare cause Blood O can be put in blood AB if you be careful enough maybe
I hope it help
State three factors affecting breathing rate in human beings
Answers
Answer: age, smoking habits, and size/weight
Explanation:
The older you get, the weaker you'll be, so your breathing will be effected.
If you smoke then your lungs will be effected which will ultimately have an affect your breathing.
If you're really heavy (ex. Over weight) then you will get tired more easily, which will effect your breathing rate.
The fluid mosaic model of the membrane proposed that membranes
Answers
Answer:
constant of protein hope it helps you
national game of china
Answers
Answer:
the national game of China is
Explanation:
ping pong which is also known as table tennis
which type of body habitus routinely requires that the image receptor be placed lengthwise rather than crosswise
Answers
The Hypersthenic body habitus is the type that requires the image receptor to be placed lengthwise due to its broader dimensions.
The type of body habitus that routinely requires the image receptor to be placed lengthwise rather than crosswise is the hypersthenic body habitus.
A hypersthenic body habitus refers to individuals with a larger, broader body build. This body type is characterized by a wide thorax, short upper body, and a higher diaphragm. When performing radiographic imaging on a person with a hypersthenic body habitus, the image receptor needs to be placed lengthwise to accommodate their larger body size and width.
In summary, the hypersthenic body habitus is the type that requires the image receptor to be placed lengthwise due to its broader dimensions. This adjustment ensures proper imaging of the patient's anatomy, allowing for accurate diagnosis and treatment.
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