identification of deep-intronic splice mutations in a large cohort of patients with inherited retinal diseases

Answers

Answer 1

Mutation is the causative factor leading to (Inherited Retinal Diseases) IRDs. But, there sequencing remains a issue due to their position, which is non coding regions. Generally the coding regions are targeted for sequencing. 

So, authors Qian et al., 2021 used Whole genome sequencing (used in Human Genome Project also) to understand the role of deep intronic splice variants to IRD. They identified 6 harmful variants which were further validated by minigene system, by checking the splicing patterns. The assigned prediction scores to the disrupted splice site was seen to be correctly associated with changed mRNA formation. Thus, authors conclude that deep intronic sites should be further researched for further understanding and development of treatment methods. 

Splice site refers to the region between the exon and intron that is responsible for splicing of codon into correct sequence for formation of protein. Variation in splicing will result in abnormal protein formation, through defected mRNA, subsequently leading to Inherited Retinal Diseases. Studies on these variants can provide cure of this existing eye problem.

Learn more about retinal diseases -

https://brainly.com/question/942982

#SPJ4


Related Questions

questions.
Questions
1 a Name the microorganism which causes the disease smallpox.

Answers

Answer: Variola Virus

Explanation:

Have you ever experienced swollen lymph nodes when you were sick? Why might this happen?

Answers

Answer:

Yes, swollen lymph nodes are more common than some might think.

Explanation:

The medical terms for swollen lymph nodes are adenopathy or lymphadenopathy.

Lymph nodes play a vital role in fighting off sickness in the body. They act as a filter, trapping bacteria and viruses before they can spread too far.

Lymph nodes typically swell up as a response to bacteria or viruses. Though cancer can be a factor in swelling, it is very rare. Most commonly they are felt right below the corner of the jaw, in the neck. Yet, they are not only in the neck; they are all throughout the body. When they are swollen, they might feel tender or even painful.

binding of the signaling molecule epinephrine to its receptor results in * 10 points a) increased concentrations of cyclic amp in the cytoplasm. b) lower blood glucose levels. c) activation of a steroid hormone receptor inside muscle cells. d) inactivation of the enzyme glycogen phosphorylase.

Answers

The binding of the signaling molecule epinephrine to its receptor results in inactivation of the enzyme glycogen phosphorylase

What are signaling molecules?

As a broad term for molecules that particularly attach to other molecules, ligand is frequently used to refer to signaling molecules (such as receptors). The message sent by a ligand is frequently transmitted inside the cell through a series of chemical messengers.

What is the role of epinephrine as a signaling molecule?

Epinephrine is an important cell signaling molecule in the fight or flight response.Also known as adrenaline, epinephrine is an efficient messenger that signals many cell types throughout the body with many effects. In the lungs, epinephrine binds to receptors on smooth muscle cells wrapped around the bronchioles.Through the activation of PKA, cAMP, and 2-adrenergic receptors, epinephrine induces the phosphorylation of GS at sites 2, 1a, and 1b (5, 36). Additionally, PKA upregulates GS phosphorylation via phosphorylating RGL, a glycogen-targeting regulatory component of protein phosphatase-1 (PP1).

Hence, binding of epinephrine to the receptor results in the inactivation of enzyme glycogen phosphorylase

To learn more about signaling molecules click on the link

https://brainly.com/question/1600575

#SPJ4

1.Why do scientists pick up ash and lava?
to be dangerous
to be cool
to study them
to clean up

Answers

Answer:

To study them.

Explanation:

They take ash and lava to study the chemicals and stuff that makes it up.

which type of adipose tissue has a greater number of mitochondria in its cells?

Answers

Brown adipose tissue has a greater number of mitochondria in its cells compared to white adipose tissue

Brown adipose tissue contains a higher density of mitochondria, which contributes to its brown color and its ability to generate heat through thermogenesis. While white adipose tissue is specialized for energy storage, brown adipose tissue has a high concentration of mitochondria and uniquely expresses uncoupling protein 1, enabling it to be specialized for energy expenditure and thermogenesis. White adipocyte cell is classically spherical, it is full of single lipid droplets and contains few mitochondria. Brown adipocyte is usually smaller than white and beige ones. It contains a large number of mitochondria and contains multiple small lipid droplets.

Learn more about Brown adipose tissue here: https://brainly.com/question/29820863

#SPJ11

25 POINTSSSS!!!!!
In which system of plant do the tissues xylem and phloem work?

Answers

In vascular system of plant do the tissues xylem and phloem work

Xylem cells are non-living cells that are part of a plant's vascular or conducting tissue. Xylem transports water from the roots upward through the plant, along with nutrients. Xylem is found in vascular bundles, that can be arranged in a circular fashion or a scattered fashion in woody and non-woody parts of plants with wood. The veins found in leaves, stems and roots which are interconnected contain xylem. Phloem, also part of the vascular tissue, conducts nutrients to parts of the plant where needed, particularly sugar produced by photosynthesis. Phloem cells are living and transport sap from the leaves, to roots or to storage areas in a plant such as a bulb. Phloem sap moves from sugar sources to sugar sinks due to turgor pressure gradient. CCredit: Enotes.com internet source!

The evidence exists in a variety of categories, including direct observation of evolutionary change, the fossil record, homology, and biogeography Sort the following examples into the correct categories. Drag each phrase to the appropriate bin. View Available Hint(s) Reset Help smlarity of endemic sland species to nearby mainiand species vestigial pevis in the high concentration of same genetic code in right whalesnearby mammalan forelimbs refies and tobacco plants n Australia development of drug dscovery of shels disovery of transitionl resistance in bacteriaef extinct species forms ofl horses blogeography homology direct observation of evolutionary change fossil record

Answers

The correct categories for the given examples are as follows:
1. Direct observation of evolutionary change:

i. Development of drug resistance in bacteria

ii. Vestigial pelvis in right whales

2. Fossil record:

i. Discovery of transitional forms of horses

ii. Discovery of shells of extinct species

3. Homology

i. Same genetic code in fireflies and tobacco plant

ii. Similarities in mammalian forelimbs

4. Biogeography:

i. the high concentration of marsupials in Australia

ii. Similarity of endemic island species to nearby mainland species

Fundamental issues about adaptation, including as its pace, temporal dynamics, and underlying phenotypic and genomic architecture, can be answered directly by observing evolution in response to real environmental change.

Paleontologists, archaeologists, and geologists use the fossil record to put significant occurrences and species in the right geologic epoch. It is based on the Law of Superposition, according to which the lower layers of undisturbed rock sequences are older than the upper levels.

The basic idea of evolutionary homology is simple to explain: homologies are characteristics that are present in and passed down from a common ancestor, and are comparable in structure and position in two or more organisms.

The study of geographic distribution patterns of organisms and the variables that influence such distributions is known as biogeography. This field is crucial to our comprehension of the evolution and adaption of marine mammals.

To know more about evolutionary change here:

https://brainly.com/question/15123048#

#SPJ11

Which of the following conditions result in the highest rate of transpiration?
A warm, windy and dry
B warm, windy and wet
C cold, windy and dry
D warm, still and wet

Answers

Answer:

the answer is A

a. What is a pH buffer and how does it work?

Answers

Explanation:

A buffer is an aqueous solution that can resist significant changes in pH levels upon the addition of small amount of acid or alkali. Each buffer is characterized by a set ‘capacity’ which is defined as the quantity of strong acid or base that must be added to change the pH of one liter of solution by one pH unit. In other words, buffer capacity is the amount of acid or base that can be added before the pH begins to change significantly.

A buffer range is the specific pH range in which a buffer effectively neutralizes the added acid or base, while maintaining nearly constant pH. Capacity and range of a particular buffer ensures that the added small amount of acid/base is neutralized and the chemical reaction keeps going without giving a wrong outcome for the experiment/process. Simply put, a buffer is a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid

When you combine sulfuric acid with sugar, you will obtain a reaction. Watch the video segment "Candy
Shop" with the sound off. This video segment shows the reaction between sulfuric acid and sugal. Describe
when
you know that the initial chemical reaction has taken place, and describe further changes and how you
know that they are occurring.​

Answers

Answer:

This question is incomplete

Explanation:

This question is incomplete because of the absence of the video. However, the addition of sulphuric acid (H₂SO₄) to sugar produces what is known as sugar charcoal through the process of dehydration of the sugar. The process described below should be similar to what is in the video as the process is the same and provides the same result.

When the acid is added, there is a change in the colour of the sugar from white to brown (evidence of initial reaction) and then subsequently black (further change). As the reaction continues, there is an observable increase in size of the sugar (further change and how we know reaction is occurring).

Which of the following organelles is responsible for creating ribosomes?

Answers

Answer:

Nucleolus

Explanation:

The nucleolus is an organelle within the nuceus that begins the assembly of ribosomes

Please help...Will mark brainiest....It is due and I need help

Please help...Will mark brainiest....It is due and I need help

Answers

Answer:

Explanation:

For the 1st one with the Cheetah: It would be a long tail because it helps them balance when they turn. Sinch Cheetahs move extremely quickly they need a balance to ensure they don't topple over when they turn, this tail provides this balance.

For the 2nd one with the bear: Thick coats on a bare will help provide warmth whenever they go to hybernate. Thin fur would leave room for freezing and wouldn't be able to help them maintain the proper body temperature.

The 3rd one with the clownfish: A round tail provides a bigger boost when swimming since it is able to push more water. A forked tail would create a gap when they swim so they would have to expend more energy than with a round tail.

The 4th one with the Finch: Since grubs live underground a thinner beak would be able to dig them out and stab them faster. This allows them to reach farther and get more food.

Answer:

D AZ FCSDF SDF F

Explanation:

just seeing how this works don't mind me

What is the most appropriate way to present the data below?
Week
1
2
Plant Mass (g)
22
61
AWN
87
123
O bar graph
chart
line graph
O pie graph

Answers

Answer:

Line Graph

Explanation:

It would be easier to see the data in a line graph. This way, you could easily see the growth of mass and the (i'm guessing height?) of the AWM in relation to the time (aka week). The bar graph could work, but a line graph would be a better option for this information.

Radiocarbon (C14) dating of organic samples is:
a relative dating technique.
an absolute dating method.
an absolute dating method used on samples up to about 2.5 million years old.
a biochemical technique for studying evolutionary relationships.

Answers

Radiocarbon (C14) dating of organic samples is an absolute dating method used on samples up to about 2.5 million years old.

Radiocarbon dating is a technique used to determine the age of organic materials based on the decay of the radioactive isotope carbon-14 (C14). This method is considered an absolute dating technique because it provides a numerical age estimate for the sample being analyzed.

Organic materials, such as bone, wood, and charcoal, contain carbon, including a small fraction of radioactive carbon-14. When living organisms are alive, they take in carbon-14 from the environment through processes like photosynthesis or consumption of other organisms. After death, the amount of carbon-14 in the organism gradually decreases over time as it undergoes radioactive decay.

By measuring the remaining amount of carbon-14 in a sample and comparing it to the known decay rate, scientists can calculate the age of the sample.

Learn more about absolute dating here:

https://brainly.com/question/26296076

#SPJ11

Why water is a wonder liquid? Justify ( A major resource to determine life on the earth.)


please please guys give me answer of this Question​

Answers

Answer:

Following are the reasons:

Water is a major solvent in nature.Water is a major solvent in nature.Water allows materials to sit upon it for pollen, dust, water insects, and other materials.Water is a major solvent in nature.Water allows materials to sit upon it for pollen, dust, water insects, and other materials.Wate is essential for daily activities and drinking.Water is a major solvent in nature.Water allows materials to sit upon it for pollen, dust, water insects, and other materials.Wate is essential for daily activities and drinking.Most of the chemical reaction is only possible due to water.Water is a major solvent in nature.Water allows materials to sit upon it for pollen, dust, water insects, and other materials.Wate is essential for daily activities and drinking.Most of the chemical reaction is only possible due to water.Living organism animals, plants, microorganisms, human beings mainly contain water that makes life possible on the earth.Water is a major solvent in nature.Water allows materials to sit upon it for pollen, dust, water insects, and other materials.Wate is essential for daily activities and drinking.Most of the chemical reaction is only possible due to water.Living organism animals, plants, microorganisms, human beings mainly contain water that makes life possible on the earth.Environment temperature, rainfall etc are maintained by water.Water is a major solvent in nature.Water allows materials to sit upon it for pollen, dust, water insects, and other materials.Wate is essential for daily activities and drinking.Most of the chemical reaction is only possible due to water.Living organism animals, plants, microorganisms, human beings mainly contain water that makes life possible on the earth.Environment temperature, rainfall etc are maintained by water.There are much more other properties which make water as a wonderful liquid.

helpppppppppppppppppp

helpppppppppppppppppp

Answers

Using radio telescopes, astronomers have found evidence the new matter is forming in the universe, which is what the big bang theory predicted.

Your answer is B

Well since the big bang theory already supports the idea of new matter forming, we are using new technology to prove an existing theory

In this case, we are using new technology to prove existing theories. NOT making new ones, or modifying them.

amin acids are used to build

Answers

Answer:

PROTEINS!

Explanation:

Amino acids, often referred to as the building blocks of proteins, are compounds that play many critical roles in your body. They're needed for vital processes like the building of proteins and synthesis of hormones and neurotransmitters.

Answer:

Proteins

Explanation:

Amino acids are the monomers, or the building blocks of proteins. There are 20 different amino acids that can make up proteins. The ribosomes in a cell perform protein synthesis and create proteins by assembling the amino acids, according to the DNA's genetic code. The proteins created are used for structure, growth, repair, maintaining pH, balancing fluids and more. In addition, they can be used as enzymes and hormones.

The more classification levels that two organisms share,

A) Group of answer choices

B) The easier it is to tell them apart

C) The more directly related they are

D) They closer together on Earth they live

The more twins they are

Answers

Answer:A or the more twins they are

Explanation:

where does glycolysis take place in a cell?

Answers

Glycolysis takes place in the cytoplasm. Within the mitochondrion, the citric acid cycle occurs in the mitochondrial matrix, and oxidative metabolism occurs at the internal folded mitochondrial membranes (cristae).

Help me with question 2 quick please!!!

Help me with question 2 quick please!!!

Answers

In a pedigree, circles typically represent female individuals, while squares represent male individuals. Pedigrees are diagrams that illustrate the genetic relationships within a family across multiple generations. They are commonly used in genetics to study the inheritance of traits or genetic disorders.

The circles and squares in a pedigree represent individuals within the family, with the shapes denoting their gender. The lines connecting the shapes indicate relationships, such as marriages or parent-child connections. Offspring are typically shown below and connected to their parents.

Therefore, by analyzing a pedigree, geneticists can study patterns of inheritance, track the transmission of genetic traits or diseases, and make predictions about the likelihood of specific traits or conditions appearing in future generations.

For more details regarding pedigree, visit:

https://brainly.com/question/13667608

#SPJ1

Intensive fishing is one factor that contributes to algae overgrowth why?

Answers

It allows more sunlight to be available to algae increasing their growth.

Oil is a fossil fuel that provides power to sustain a modern lifestyle. Which of these statements best describes a cost of oil drilling? oxygen gas is absorbed. Oceans and rivers dry up. Radiation may cause health problems. Water and soil may be contaminated.

Answers

The statement that best encapsulates the cost of oil drilling is "water and soil may be contaminated."

How is fossil fuel utilized?

Petroleum-based products are used in the production of electricity, building heating, and vehicle propulsion. Plastics, polyurethane, solvents, and countless other intermediate and finished goods are produced by the petrochemical industry in the industrial sector using petroleum as a raw material (a feedstock).

Because they are mixtures of hydrocarbons that formed from the remains of plants and animals (diatoms) that lived in a marine environment millions of years ago, before dinosaurs existed, we refer to crude oil and petroleum as fossil fuels.

To know more about fossil fuel visit:

https://brainly.com/question/7971256

#SPJ4

which description is the best definition of a single nucleotide polymorphism, or snp? any variation in a single dna base between two individuals any variation in the sequence of genes that alters the morphology of an organism an rna protein in the spliceosome that marks where introns get excised during splicing events an artificially induced mutation in a protein coding region of the genome

Answers

The most accurate description of a snp, or single nucleotide polymorphism any difference in a single DNA nucleotide between two people.

Which of the following definitions most accurately describes a single nucleotide polymorphism, or snp?A single nucleotide polymorphism (SNP) is a variation in a single DNA base between two individuals.SNPs are the most common type of genetic variation and can occur in any location within a gene or the non-coding regions of the genome.SNPs are often used to study genetic differences between individuals or populations and to identify potential genetic risk factors for diseases.SNPs can result from both naturally occurring events and artificially induced mutations.When a SNP occurs in a protein coding region of the genome, it can have an effect on the function of the protein, which in turn can affect the phenotype of the organism.SNPs can also be used to trace ancestry and to identify populations that share a common evolutionary history.SNP data can be used to study the evolution and population structure of a species, as well as to identify potential gene-environment interactions.

To learn more about single nucleotide polymorphism, or snp refer to:

brainly.com/question/30135675

#SPJ4

what is the function of ribosomes in cells

Answers

Answer:

Function - Ribosomes are responsible for making protein through amino acids. The proteins created are essential to cell and organismal function. Some ribosomes are attached to the endoplasmic reticulum (rough ER), others float freely within the cytoplasm.

Explanation:

Answer:

Ribosomes are organelles that participate in protein synthesis.

Explanation:

For individual ribosomes to participate, they must be interconnected. Thus, ribosomes become active in protein synthesis only when they come into contact with mRNA.

Several individual free ribosomes bind to the mRNA that passes between their subunits and therefore forms a structure called polysome, which resembles a low bead.

The number of ribosomes in the polyribosome is determined by the complexity of the protein it synthesizes, ie the number of amino acids in the protein. Thus, for example, hemoglobin is composed of 150 amino acids, and the polysomes that synthesize this protein consist of only 5 ribosomes.

what is the genotype if the male animal

Answers

Answer a male animal Carrie’s one X chromosome received from his other and one Y chromosome received from his father

How does point mutation affect the amount of DNA?

Answers

The point mutation can alter the amount of DNA sequences depending upon the type of mutation.

A point mutation is a type of mutation in which the DNA or RNA, the organism's genetic material, is altered by the addition, deletion, or modification of a single nucleotide base. The amount of DNA is unaffected by base substitution since the nucleotide count is unaffected after the substitution. The amount of DNA grows with insertions and mutations because there are more nucleotides involved, whereas it decreases with deletion mutations. A common example of substitution point mutation is sickle cell anemia.

Hence, the type of point mutation determines the amount of DNA.

To know more about Sickle cell.

https://brainly.com/question/3895081

#SPJ4

how do prions differ from viruses?

Answers

Both viruses and prions are infectious agents, but prions are special in that they can spread their own misfolded structure and result in life-threatening neurological disorders.

To be more specific, prions are distinct in that they lack any genetic material, like DNA or RNA. Instead, they are merely aberrant forms of the prion protein, a typical cellular protein. A normal prion protein may take on the same misfolded structure when it comes into touch with a prion. The misfolded prion protein can serve as a template to transform more normal prion proteins into the misfolded form, allowing this process to continue.

Prions are harmful because of their capacity to spread their own distorted form. These can result in a number of neurodegenerative illnesses, including Creutzfeldt-Jakob disease and mad cow disease, as they build up in the brain. Prions, in contrast to viruses, are not eliminated by routine sterilising techniques, and there is no recognised treatment for prion illnesses.

In comparison, viruses are far more intricate structures with genetic material and frequently have a protein coat on the outside. By taking over the cell's mechanism to create additional virus particles, they invade cells. Unlike prions, viruses can be eliminated using a variety of techniques, including antiviral medications, vaccinations, and disinfectants.

For more such questions on prions .

https://brainly.com/question/13022105#

#SPJ11

Carbohydrate stored in the muscles of a marathon runner is converted to available glucose during a race. This conversion is
controlled by the hormone
(A)Adrenalin
(B)insulin
(C)thyroxin
(D)testosterone

Answers

(A) Adrenalin
It’s adrenalin because when you’re excited, adrenaline rushes in your body

which one of the following machines moves like a millipede?
A. tank
B. excavator
C. train
D. A and B
I can't understand this question till now.
Please answer it quickly!​

Answers

Answer:

the answer is letter A. Tank

1. Complete a dichotomous key for the 10 leaves on the Common Leaves sheet. The chart provided here allows for 11 pairs of statements. Depending on how you build your dichotomous key, you may or may not need all of them, or you may need to add some.
Statement Types/Dichotomous key for leaves Identification Name/Number of Leaves
Statement 1a Click or tap here to enter text.
go to statement or identify leaf Click or tap here to enter text.

Statement 1b Click or tap here to enter text.
go to statement or identify leaf Click or tap here to enter text.

Answers

This dichotomous key should help you identify the leaves on the Common Leaves sheet by asking a series of yes/no questions and directing you to the appropriate leaf based on your answers.

Sure! Here's a dichotomous key for the 10 leaves on the Common Leaves sheet:

1a. Leaves are needle-like or scale-like (go to Statement 2)

1b. Leaves are not needle-like or scale-like (go to Statement 3)

2a. Leaves are arranged in clusters (go to Statement 4)

2b. Leaves are not arranged in clusters (go to Statement 5)

3a. Leaves are broad and flat (go to Statement 6)

3b. Leaves are not broad and flat (go to Statement 7)

4a. Clusters of leaves have 2 needles (go to Leaf 1)

4b. Clusters of leaves have more than 2 needles (go to Leaf 2)

5a. Leaves are feathery and fern-like (go to Leaf 3)

5b. Leaves are not feathery and fern-like (go to Statement 8)

6a. Leaves are heart-shaped (go to Leaf 4)

6b. Leaves are not heart-shaped (go to Statement 9)

7a. Leaves have a sawtooth edge (go to Leaf 5)

7b. Leaves do not have a sawtooth edge (go to Statement 10)

8a. Leaves have rounded lobes (go to Leaf 6)

8b. Leaves do not have rounded lobes (go to Statement 11)

9a. Leaves have a waxy texture (go to Leaf 7)

9b. Leaves do not have a waxy texture (go to Leaf 8)

10a. Leaves have deeply cut lobes (go to Leaf 9)

10b. Leaves do not have deeply cut lobes (go to Leaf 10)

Leaf 1: White Pine

Leaf 2: Red Pine

Leaf 3: Maidenhair Fern

Leaf 4: Sweetgum

Leaf 5: Red Maple

Leaf 6: Sugar Maple

Leaf 7: Magnolia

Leaf 8: Dogwood

Leaf 9: Oak

Leaf 10: Ginkgo

For similar questions on  dichotomous key

https://brainly.com/question/2963225

#SPJ8

Other Questions
To determine Days Sales Outstanding, you do not need to know theA/R Turnover.True or False? find the complex root of 8x^2+16x+8 four guys company has in its inventory 5,000 damaged televisions that cost $50,000. the televisions can be sold in their present condition for $32,000, or repaired at a cost of $43,000 and sold for $76,000. what is the opportunity cost of selling the televisions in their present condition? Most hormones are produced by which humanorgan system?(1) digestive(2) endocrine(3) respiratory(4) nervous eory.According to cell theory, which of the following aremade of cells? Check all that apply.O flowersrocksbloodwaterbacteriasugarskinRETRY divide 240 in the ratio 3:5:12 The triplet codes in DNA needed to specify a specific polypeptide chain are found in the: cytoplasm. anticodon of tRNA. codon of mRNA. gene. It can be difficult for a president to balance the varied opinions of other Americans when making major decisions. Argue for or against this statement, using evidence from the text to support your argument. what are the goal of sport medicine professional 8. The floor of a rectangular meetingroom has a raised platform that is thesame length as the room. The wholeroom has an area of 461 square yards.Raised PlatformTS7 yards22/2Part AWhat is the total width of the room?Write an equation to model your work.70476/133704152Part B45Suppose the area of the platform is73 square yards. What fraction of theentire floor is the raised platform?Write an equation to show your work How do you write the chemical symbols for isotopes? Where is probability, data, trends, likelihoods and/or statistics used in veterinarian? Be specific and explain. -5 2/3 - 1/6 please help Can someone plz help me? The Emergency Banking Act empowered the secretary of the treasury to decide which banks could safely be reopened and: Underline the line that best illustrates how the death of Curly impacted Buck. Explain. FILL THE BLANK. customers do not expect blank______ products in full-line discount stores. Solve the following to the problem Show the complete solutions of the following problem below:The first term of a geometric sequence is 3 and the ninth term is 768. Find the common ratio of the sequence and the value of a12.The common ratio of a geometric sequence is 4 and its 5th term is 192. Find the value of the first term and determine the 9th term of the sequence. hich of the following represents a legitimate increase in LTC policy premiums for existing insureds? a. Insurer A issued a premium increase notice for anyone whose claims experience had been 10 percent higher than originally anticipated. b. Insurer B raised the premiums of all its insureds in a particular city whose residents filed twice as many claims per capita than any other city. c. Insurer C raised the premiums of Lester's policy because he has shown symptoms of cognitive failing. d. Insurer D increased renewal premiums for all its insureds who are age 71 and over