calculate the mass in g of agarose required to make 200 ml of a 1.2% agarose gel. record the answer to one decimal place

Answers

Answer 1

The mass in g of agarose required to make 200 ml of a 1.2% agarose gel is 2.4 g.

Agarose gel is defined as a three-dimensional matrix which is formed from helical agarose molecules in supercoiled bundles that are aggregated into three-dimensional structures with channels and pores through which biomolecules can pass.

Agarose gel electrophoresis is the main use of agarose gel which has proven to be an efficient and effective way of separating nucleic acids.

1.2% refers that 1.2 g of agarose is required to make 100 ml of agarose gel.

So, 200 ml of agarose gel will require 2.4 g of agarose.

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Related Questions

What is the molarity of a solution that contains 4.53 moles of lithium nitrate (LiNO3) in 2.85 liters of solution

Answers

Answer:

M= ml - 45301 - 11.59M.

Explanation:

3.Calculate the Standard deviations of the following and round the final number to one less decimal place:a.C02: 33, 37, 40, 42, 45, 30, 25, 35, 47b.Chloride: 100, 102, 97, 95, 88, 90c.Sodium: 150, 148, 160, 157, 135d.Potassium: 4.5, 6.0, 5.5, 3.7, 4.0, 2.7, 2.0, 2.3, 2.5, 3.6

Answers

Answer

a. CO₂: the standard deviation of the set is 6.76

Convert 11.9 g of Be(NO2)2 to moles.

Answers

To convert grams (g) of a substance to moles (mol), you need to divide the mass by the molar mass of the substance.

The molar mass of Be(NO2)2 can be calculated by adding the molar masses of its constituent atoms, which are:

The molar mass of Be: 9.01 g/mol

The molar mass of N: 14.01 g/mol

The molar mass of O: 16.00 g/mol (there are two oxygen atoms in the nitrite ion, NO2-)

So the molar mass of Be(NO2)2 is:

9.01 g/mol (Be) + 2(14.01 g/mol (N) + 2(16.00 g/mol (O)) = 9.01 g/mol + 28.02 g/mol + 32.00 g/mol = 69.03 g/mol

Now we can use this molar mass to convert 11.9 g of Be(NO2)2 to moles:

moles = mass/molar mass

moles = 11.9 g / 69.03 g/mol

moles ≈ 0.1727 mol

Therefore, 11.9 g of Be(NO2)2 is equivalent to approximately 0.1727 moles.

What are the possible values of 1 and m for
n=4 ?​

Answers

Answer:

If n = 4, then the possible values of 1 and m depend on the equation or expression being used. Without more information, it is impossible to determine what the possible values of 1 and m might be. Can you please provide more context or information about the problem you are trying to solve?

When the hydronium ion concentration of a solution is increased
by a factor of 10, the pH value of the solution
(1) decreases 1 pH unit
(2) decreases 10 pH units
(3) increases 1 pH unit
(4) increases 10 pH units

Answers

Answer:

The correct answer is:

(1) decreases 1 pH unit

Our 4 seasons are caused by

Answers

Answer: Earth and the sun

Explanation:

Because the earth  is tilt toward the sun the planet rotates about axis but it isn't visible,also at different times of the year the northern ans southern axis are close to the sun.

hope this helps  

If a small pig runs 46 meters in 6 seconds, what is the pig's average speed?
6 m/s
276 m/s
7 m/s
48 m/s

Answers

Answer:

the pig's average speed is 7 m/s

The average speed of a moving body is the ratio of the distance travelled to the time of travel. Here, the speed of the pig running 46 meters in 6 seconds is 7.6 m/s.

What is average speed ?

The speed of an object is a physical quantity that measures the distance travelled by the object per unit time. Hence, it is the ratio of distance travelled to the  time taken for the travel. The rate of speed is called velocity. Bot are having the standard unit of m/s.

Given that,

distance covered by the pig = 46 meters

time taken = 6 seconds

speed  = distance/time.

Average speed of the pig = 46 meters/ 6 seconds = 7.6 m/s.

Therefore, if the small pig runs 46 meters in 6 seconds, it is having an average speed of 7.6 m/s.

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what is the pH of a 8.27*10^-2 M solution of HClO_4

Answers

Answer: 0.0827

Hope This Will Help You And This Should Be The Correct One.

How do you determine the volume of an irregular object if you don't know the density?
to determine the mass of an object?

Answers

I think the answer is a not sure tho hope u the answe

An organized method scientists use to solve problems and test

hypotheses
hypothesis
scientific process
conclusion
experiment

Answers

Explanation:

Hypothesis is answer.

Have a great day

The end point of a titration was reached after 23.1 mL

of 0.050 M

disodium EDTA titrant was dispensed into a solution containing the zinc ion. Calculate the moles of disodium EDTA used.

Answers

The moles of disodium EDTA used be 0.001155 moles.

What is meant by disodium EDTA?

Ethylenediaminetetraacetic acid has a salt known as disodium EDTA (commonly known as EDTA). It serves largely as a chelating agent in cosmetic formulations, preventing chemicals from forming bonds with trace elements (mostly minerals) that may be present in water or other components.

In the structure of EDTA, there are typically four negatively charged oxygen atoms. These two oxygen atoms are joined by two sodium cations to form disodium EDTA. An unwanted byproduct of EDTA production is disodium EDTA. Ethylenediamine, formaldehyde, and sodium cyanide are components in the manufacture of EDTA.

The following formula is used to determine how many moles of disodium EDTA are needed.

Moles = molarity × volume in liters

Molarity = 0.050m

Volume in liters = 23.1/1000 = 0.0231 L

Moles =  0.0231 x 0.050 = 0.001155 moles of disodium EDTA

Therefore, the moles of disodium EDTA used be 0.001155 moles.

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What is the molarity of a solution containing 2 moles of sodium hydroxide (NaOH) dissolved in 4.0 L of solution?

Answers

Answer:

0.5M

Explanation:

Molarity = Mols/ Liters

so 2/4

0.5 M

Write a balanced chemical equation for the following reaction in an acidic solution: Copper metal reacts with aqueous nitric acid to form aqueous copper (II) nitrate, liquid water and gaseous nitrogen monoxide.

Answers

Answer:

Cu(s) + 4HNO₃(aq) -> Cu(NO₃)₂(aq) + 2NO₂(g) + 2H₂O(l)

How many elements are in a isotope?

Answers

Answer:i believe 9-?

Explanation:

boron-10

boron-11

carbon-12

carbon-13

oxygen-16

oxygen-17

oxygen-18

chlorine-35

chlorine-37

There are a number of factors that alter the bioavailability of minerals from foods. Complete each sentence with either increases or decreases.
decreases increases
Phytic acid ______ the absorption of zinc from grains.
Oxalic acid _____ the absorption of calcium from spinach.
Refinement of grains _____ the mineral content of grain products.
Vitamin C _____ the absorption of iron from foods.
Vitamin D ____ the absorption of calcium from foods.

There are a number of factors that alter the bioavailability of minerals from foods. Complete each sentence

Answers

Each sentence about the bioavailability of minerals in foods should be completed with either increases or decreases as follows:

Phytic acid decreases the absorption of zinc from grains.Oxalic acid decreases the absorption of calcium from spinach.Refinement of grains decreases the mineral content of grain products.Vitamin C increases the absorption of iron from foods.Vitamin D increases the absorption of calcium from foods.

What are nutrients?

Nutrients can be defined as the chemical compounds (substances) or minerals that are present in food, and they are needed by the body of a living organism for healthy growth, reproduction, development, proper functioning, and reproduction.

What are the classes of nutrients?

In Science, there are five (5) main classes of nutrients and these include the following:

ProteinFatCarbohydrateVitaminsMinerals

In conclusion, phytic acid, oxalic acid, and refinement of grains are factors that decrease the bioavailability of minerals in foods while Vitamin C and Vitamin D increases the bioavailability of minerals in foods.

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sb+I2 rendimenti and reagent ​

Answers

hindi ko po getssssss

yan pasyensya na po

What would be the final value for the enthalpy CO2+2h2o h =-1410 Kj

Answers

The final value for the enthalpy change of the formation of CO2 and 2H2O from their elements (C, H2, and O2) would be -1410 kJ per mole of CO2 and 2 moles of H2O formed.

The enthalpy change (ΔH) for the reaction CO2 + 2H2O → H2CO3 can be calculated by multiplying the stoichiometric coefficients of the balanced equation by the enthalpy values of the corresponding compounds involved in the reaction.

In the given reaction, the enthalpy change is -1410 kJ. However, it's important to note that this enthalpy change corresponds to a specific reaction and may not directly apply to the formation of CO2 and 2H2O from another reaction or process.

If we assume that the reaction is the formation of one mole of CO2 and two moles of H2O, we can say that the enthalpy change for this specific formation reaction is -1410 kJ.

Therefore, the final value for the enthalpy change of the formation of CO2 and 2H2O from their elements (C, H2, and O2) would be -1410 kJ per mole of CO2 and 2 moles of H2O formed.

It's worth mentioning that the enthalpy change can vary depending on the specific conditions (temperature, pressure, etc.) and the reactants involved in the reaction. Therefore, it's crucial to specify the conditions and reaction context when referring to enthalpy values.

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is energy from the light source conduction convention or radiation​

Answers

Energy from a light source is known as radiation.

We can see each other in the classroom right now because we
a.reflect visible light
b. absorb visible light
c.reflect infrared light
d.absorb radio waves.

Answers

I’m pretty sure it’s B but i’m not sure

Answer:

The answer is A

Reflect visible light

How many moles of Ne are contained in a 5.00 L tank at 155°C and 2.80 atm?

Answers

There will be 0.39 moles

Take a look at the He gas in the tank:

P = 2.80 atm, or pressure

Volume = 5.00 liters.

T = (273 + 155) is the temperature. K = 428 K

R = 0.08206 L atm/L of gas (mol K)

Charle's law, Boyle's law, and Gay-Lussac law are the three gas laws that make up the combined gas law, commonly referred to as a general gas equation. For a certain amount of gas, the law illustrates the relationship between temperature, volume, and pressure.

Gas law PV = nRT

So, n = PV/. (RT)

He gasses number = 2.80 x 5.00 / (0.08206 x 428) mol equals 0.39 mol.

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please help I need this finished asap. Please put answers down below.

please help I need this finished asap. Please put answers down below.
please help I need this finished asap. Please put answers down below.

Answers

Image 1

1) There is more proton repulsion in Uranium than in Barium

2) Barium experiences a stronger force holding the nucleons together

3) The repulsion of the neutrons in Uranium makes the nuclide unstable

4) The higher the atomic number, the more likely that the nucleus is radioactive.

5) U-238 has 146 neutrons

6) U - 235 has 143 neutrons

Image 2;

1) You can minimize background radiation by limiting your outdoor exposure.

2) Cosmic radiation is the most difficult to avoid.

What is background radiation?

The natural radiation that is constantly present in the environment is known as background radiation.

It includes internal radiation found in all living things as well as cosmic radiation, which originates from the sun and stars and terrestrial radiation, which originates from the Earth.

We know that the repulsion of the protons in the Uranium nucleus is more than that of the barium nucleus because there are more neutrons in the Uranium nucleus. It is this repulsion that makes Uranium unstable.

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HELP PLEASE PLEASE PLEASE. Can anyone tell me how to separate the following mixture
A) ethanol in water
B) boiling the mixture of chloride crystals with water
C) pure water from muddy water
D) sodium chloride in water
E) sodium carbonate in water
F) chlorophyll from leaves
G) mixture of acetic acid and alcohol
H) serum from blood sample
I) kerosene from water
J) ammonium chloride in sand
I NEED CORRECT ANSWERS ONLY.
HURRY UP PLEASE. I WILL MARK AS BRAINLIEST

Answers

A) Ethanol in water: Distillation.

B) Boiling the mixture of chloride crystals with water: Evaporation.

C) Pure water from muddy water: Filtration.

D) Sodium chloride in water: Evaporation or Crystallization.

E) Sodium carbonate in water: Filtration or Evaporation.

F) Chlorophyll from leaves: Extraction using a suitable solvent like ethanol.

G) Mixture of acetic acid and alcohol: Distillation.

H) Serum from blood sample: Centrifugation.

I) Kerosene from water: Separatory funnel or Decantation.

J) Ammonium chloride in sand: Sublimation or Dissolving in water and Filtration.

A) Ethanol in water: Distillation can be used to separate ethanol from water based on their different boiling points.

B) Boiling the mixture of chloride crystals with water: By heating the mixture, the water will evaporate, leaving behind the chloride crystals.

C) Pure water from muddy water: Filtration can be used to separate the solid particles (mud) from the water.

D) Sodium chloride in water: Evaporation can be used to separate sodium chloride from water by heating the mixture until the water evaporates, leaving behind the salt.

E) Sodium carbonate in water: Filtration can be used to separate solid sodium carbonate from water, similar to muddy water.

F) Chlorophyll from leaves: Extraction using a suitable solvent like ethanol or acetone can be used to separate chlorophyll from leaves.

G) Mixture of acetic acid and alcohol: Distillation can be used to separate the mixture based on their different boiling points.

H) Serum from blood sample: Centrifugation can be used to separate the serum, which is the liquid part of blood, from the solid components like cells.

I) Kerosene from water: Separatory funnel or decantation can be used to separate the immiscible liquids by pouring off the top layer (kerosene) from the bottom layer (water).

J) Ammonium chloride in sand: Sublimation can be used to separate ammonium chloride by heating the mixture, causing the ammonium chloride to vaporize and then condense back into solid form in a cooler region, leaving the sand behind.

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you dissolve 0.01 moles of glycine in 1 liter of water and begin to titrate the resulting solution using concentrated naoh (insignificant volume added).a. Draw the titration curve in terms of pH vs equivalents of base. Locate and label any buffering regions that may be present.b. You adjust the pH to 7.0. After that you add 0.005 moles of NaOH. Draw the structure(s) of the species ofglycine present in the solution and indicate the proportion of each species.c. What is the approximate pH of the solution in part b?d, Would this solution be a good buffer?

Answers

The pH of the solution, which is set at 3, depends on the kinds and amounts of glycine present in the solution as well as the pka of the NH2 group, which ranges from 9 to 78. pH = equivalent = 9.78, thus

What is glycerine's advantage?

Glycerine, an amino acid, provides a wide range of incredible health benefits. Your body needs glycerine to make essential compounds including glutathione, creating, and collagen. This amino acid may also protect your liver from the negative effects of alcohol while improving your heart health and sleep.

who shouldn't consume glycerin?

A single amino acid should not be consumed in large amounts over a lengthy period of time. Avoid consuming isolated amino acids in large amounts without first talking to a doctor. Glycine supplements shouldn't be taken by women who are pregnant or breastfeeding.

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What system is used to help match DNA from crime scenes to a DNA database?

A.
CODIS

B.
AFIS

C.
NIBIN

D.
FBI

Answers

A.CODIS (Combined DNA Index System)

Answer:

A. CODIS

Explanation:

The Combined DNA Index System (CODIS), administered by the FBI, maintains DNA profiles obtained through federal, state, and local DNA sample collection programs, and makes this information available to law enforcement agencies across the country for law enforcement identification purposes.

The pressure of a gas will ? when the volume is decreased and will ? when the absolute temperature is decreased.

Answers

Answer:

Explanation:

Pressure and volume are indirectly related.

Pressure and temperature are directly related.

so first ? is increase

2nd ? is decrease

2 facts about the characteristics of S.O.H.O

Answers

Answer:

Explanation:SOHO is a three-axis stabilised spacecraft that constantly faces the Sun. Its design is based on a modular concept with two main elements: the payload module, housing the 12 instrument packages, and the service module, providing essentials such as thrusters, power and communications.

The density of aluminium is 2.7 g/cm3. Find the mass in grams of a bar of aluminum measuring 1.7 cm by 3.0 cm by 12.9 cm.

Answers

Answer: 177.23 g.

Explanation:

the volume of the aluminum bar is

1.7 cm x 3.0 cm x 12.9 cm

= 65.61 cm^3

2.7 g/cm^3 x 65.61 cm^3

177.23g

If 12.8 g lead(II) sulfate (303.3 g/mol) precipitates when excess potassium chloride is added to 1.65 L of a water sample, what is molar concentration of Pb2+ in the sample?

Answers

Answer:

\(M=0.0256M\)

Explanation:

Hello,

In this case, the undergoing chemical reaction is:

\(PbSO_4(aq)+2KCl(aq)\rightarrow PbCl_2(s)+K_2SO_4(aq)\)

In such a way, since all the lead (II) is converted due to the excess of potassium chloride, the moles of lead (II) in the sample are computed from the mass of lead (II) sulfate:

\(n_{Pb^{2+}}=12.8gPbSO_4*\frac{1molPbSO_4}{303.3gPbSO_4} *\frac{1molPb^{2+}}{1molPbSO_4} \\\\n_{Pb^{2+}}=0.0422molPb^{2+}\)

Thus, since volume of the solution is 1.65 due to the fact that the addition of the reactants is not enough to significantly modify the reaction's volume, the resulting molar concentration of the lead (II) ions is:

\(M=\frac{n_{Pb^{2+}}}{V}=\frac{0.0422molPb^{2+}}{1.65L}\\ \\M=0.0256M\)

Regards.

Lucy had two samples of different iron ores, haematite and magnetite.
The haematite sample weighed 5 g and contained 3.5 g of iron. The magnetite sample weighed 10 g and contained 7.2 g of iron. Which of the two iron compounds contains the larger proportion of iron? show working out.

Answers

Answer:

magnetite

Explanation:

proportion of iron in haematite = 3.5/5 x 100% = 70%

proportion of iron in magnetite = 7.2/10 x 100% = 72%

Question 9
5 pts
Which energy transfer is correctly matched?
Fusion in the sun: chemical to light and heat
Lighting a match: heat energy into chemical energy
a cell phone: thermal to electrical
Turning on a ceiling fan: electrical to mechanical

Answers

Answer:

fusion in the sun

Explanation:

I know this because I am currently talking this test and I know it cause I did it

Answer:Turning on a ceiling fan: electrical to mechanical

I took the test and Fusion in the sun: chemical to light and heat Lighting a match: sound energy into chemical energy were the wrong answers.

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Are the following molecules enantiomers, diastereomers, or the same? NH NH CHIH F enantiomers diastereomers same A Hu CI F Simplity38+5.5 Answers please Q1. What is the enthalpy change during the process in which 100.g of water at 50.0C is cooledto ice at -30 C? Show your work to receive full credit. Specific heat of fusion of water = 6.01kj/mol. Specific heat of ice = 2.03 J/g c. In addition to rotating drums, clutch packs may be installed ____.All of the above.behind the front pumpon the center supportat the rear of the case Which of the following is a reason why many salespeople join civic and professional organizations, country clubs, or fraternal organizations? a) To learn the technique of cold canvassing; b) To learn about sales ethics; c) To receive sales training; d) To gain leads through networking; e) To engage in social selling How can a general chart for physical activity help patients improve their overall health? Which results from an increase in the greenhouse effect? 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The Strait of Magellan connects the Atlantic Ocean with the __________.A.Amazon RiverB.Gulf of MexicoC.Pacific OceanD.Caribbean Sea What level includes differentpopulations that live together in acertain area?A. population indexB. communityC. species Cul es la propiedad de - 53 + 53 = 0 ? A 60-watt light bulb uses 60 watt hours of electricity in 1 hour. Suppose everyone in theUnited States left one unneeded 60 watt light bulb on for one hour every day for a year.9. Electricity is billed in kilowatt hours. So 60 watt hours is equal to sixtydivided by one thousand. Express the electricity used by a 60-watt lightbulb in one hour in kilowatt hours in scientific notation.10. Express the number of days in a year in scientific notation.11. There are about 315,000,000 people in the United States. Write thatnumber in scientific notation.12. Now find how many kilowatt hours of electricity would be wasted ifevery person in the United States left on one unneeded 60-watt lightbulb one hour a day for a whole year. Express your answer in bothscientific notation and standard notation.13. The average household uses about 15,000 kilowatt hours per year.How many households could use that wasted electricity from item 12and have light for a year? Express your answer in standard notation.the original content are the responsibility o Can please post the answers for 1-5 of Use the Mole Quick Check1. The atomic mass unit is equivalent to 1/12 of the mass of a neutral atom of which element? (1 point) when a patient is admitted to the acute care floor with methicillin-resistant s. aureus,which medication would be ordered for this client? Siera Inc. had 350,000 shares of common stock outstanding throughout the year that declared and paid dividends of $50,000 during the year. The company also declared and paid dividends of $5,000 on 30,000 shares of preferred stock during the year. The company reported the forming amounts income statement for the year ended December 31 (pretax). Required a. Prepare the earnings per share section of the income statement for the year ended December 31 , assuming a tax rate of 25%. - Use a negative sign to indicate a loss. - Enter the answers for per share amounts in dollars and cents, rounded to the nearest penny. Required b. Repeat requirement of part a except now assume that the company reported income from discontinued operations of $35,000. - Use a negative sign to indicate a loss. - Enter the answers for per share amounts in dollars and cents, rounded to the nearest penny. 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