assume that you're using a recombinant yeast two-hybrid system like the one we discussed in class, using elements of the gal promoter and the lac reporter.what will happen if this system is used in cells which do not express the laci gene?if bait binds to prey, the reporter will function, but its expression level will be lower and the signal will be weaker.the reporter will be on constitutively, and it will be impossible to determine if bait has bound to prey.thre will be no expression of the reporter.nothing, because the system we discussed is induced by galactose.
Answers
To summarize, in cells that do not express the lacI gene, the reporter will be expressed in the presence of galactose due to the activation of the gal promoter. However, without the Lac repressor, the reporter may be expressed at a lower level, resulting in a weaker signal.
In a recombinant yeast two-hybrid system using elements of the gal promoter and the lac reporter, if the system is used in cells that do not express the lacI gene, there will be no expression of the reporter.
The lacI gene encodes for the Lac repressor protein, which binds to the lac operator region and inhibits the expression of the lac reporter gene. In cells that do not express the lacI gene, there will be no Lac repressor protein present to bind to the operator, allowing the reporter gene to be expressed.
However, it is important to note that the system we discussed in class is induced by galactose. So, if galactose is present in the cells, the gal promoter will be activated, leading to the expression of the reporter gene even without the Lac repressor.
This means that in the absence of the lacI gene, the reporter will still be expressed when galactose is present.
To summarize, in cells that do not express the lacI gene, the reporter will be expressed in the presence of galactose due to the activation of the gal promoter. However, without the Lac repressor, the reporter may be expressed at a lower level, resulting in a weaker signal.
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Related Questions
two animals have an identical seqence of amino acids in one of the protein found in their cells . what does this indicate ? a. they have been eating the same types of foods b. they have not been exposed to substances that cause mutation c. they must be members of the same genus d. they share an ancestor
Answers
The correct answer is d. they share an ancestor. When two animals have an identical sequence of amino acids in a protein, it suggests they share a common ancestor with a conserved genetic information.
When two animals have an identical sequence of amino acids in one of the proteins found in their cells, it indicates that they share a common ancestor. Proteins are encoded by genes, and the sequence of amino acids in a protein is determined by the DNA sequence of the corresponding gene. The DNA sequence is passed down from generation to generation through inheritance.
If two animals have the same sequence of amino acids in a specific protein, it suggests that the DNA sequence coding for that protein has been conserved over time, likely due to a shared ancestry. This conservation of the protein sequence indicates that the genetic information for producing that protein has been passed down from a common ancestor to both species.
On the other hand, options a, b, and c are not necessarily true based solely on the fact that two animals have an identical sequence of amino acids in a protein. The animals could have different dietary habits (option a), may have been exposed to different mutagenic substances (option b), and do not necessarily belong to the same genus (option c). Only the shared ancestry (option d) is directly indicated by the identical protein sequence.
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4. The cells of all organisms undergo similar processes to maintain homeostasis. What must ALL cells do in order to maintain homeostasis
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ALL cells do in order to maintain homeostasis by allows the organism in its combat to keep homeostasis.
Whst is Homeostasis ?Homeostasis is generally maintained withinside the human frame with the aid of using an incredibly complicated balancing act. Regardless of the variable being saved inside its regular range, preserving homeostasis calls for as a minimum 4 interacting components: stimulus, sensor, manage center, and effector.
Homeostasis relies upon on preserving accurate fluid stages in the cell and on changing useable materials, along with oxygen, for waste products, along with carbon dioxide.
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Can someone help me
Answers
Answer:
its c my dude
Explanation:
brainliest plz
Which level of organization includes all the other levels of organization?
organ system
organism
tissue
ОО
cell
Answers
Answer:
The answer is option b
Organism
Since all the cells, tissues and organ system come together to form an organism.
Hope this helps
What happens when someone's brain has a low level of neurotransmitters?
A. Motor neurons aren't able to send messages to muscles,
O B. Action potentials can't form in the sensory neurons.
O C. The nervous system can't create hormones,
O D. Neurons aren't able to communicate with each other.
Answers
Answer:
Neurons aren't able to communicate with each other.
Answer: D
Explanation:
What is the correct pathway that food travels during digestion? *
a. Mouth, stomach, esophagus, small intestine, large intestine
b. Mouth, esophagus, small intestine, stomach, large intestine
c. Mouth, esophagus, stomach, large intestine, small intestine
d. Mouth, esophagus, stomach, small intestine, large intestine
Answers
The correct pathway that the food travels during digestion is Mouth, esophagus, stomach, small intestine, large intestine. The correct option is D.
What is alimentary canal?The gastrointestinal tract (also called the digestive tract). This long tube of organs serves as a conduit for food to pass through the body. It includes the esophagus, stomach, and intestines and runs from the mouth to the anus (where poop comes out).
The digestive system's alimentary tract is made up of the mouth, pharynx, esophagus, stomach, small as well as large intestines, and rectum.
The alimentary tract's primary function is to convert food into absorbable particles and transport them to the body's other organs.
The correct digestive pathway is the mouth, esophagus, stomach, small intestine, and large intestine.
Thus, the correct option is D.
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scientists build models based on what they know from previous research to develop testable hypotheses. scientists watson and crick first constructed an incorrect triple-helix model of dna with the bases (a, t, c, g) arranged on the outside of the molecule. explain why their triple-helix model was valuable even though it was not correct
Answers
Triple-helix model of DNA was still valuable because they led to the development of the correct double helix model and to better understand the chemical structure and bonding of DNA.
Watson and Crick's initial triple-helix model of DNA, although incorrect, was still valuable for several reasons. First, it was a starting point for further exploration and hypothesis testing, which ultimately led to the development of the correct double helix model.
Second, the model helped scientists to better understand the chemical structure and bonding of DNA, which provided insight into how DNA functions in genetic information storage and replication.
Finally, the incorrect model helped to identify what did not work, which aided in the development of new hypotheses and experiments. In this way, even incorrect models contribute to the advancement of scientific knowledge by helping to refine understanding and identify new avenues for exploration.
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isle royale national park consists of a series of islands located in lake superior. initially, there were no wolves on any of the islands since they were in the middle of lake superior. however, in 1949, an unusually long and cold winter produced an ice bridge between canada and the islands of the national park. during this winter, a pair of wolves used the ice bridge to travel inadvertently to isle royale. today there continues to be a wolf population on the main island descended from that original pair. has allopatric speciation occurred yet with respect to the mainland wolves? how would you determine if it has occurred?
Answers
Answer:
12
Explanation:
What is the rounded portion of the incisal edge of an anterior tooth that is evident at eruption and wears with age? a. Cingulum b. Line angles c. Mamelons d. Enamel tufts
Answers
We humans are changing the composition of the atmosphere in many ways. Those changes will directly affect the planet’s temperature, but the resulting change in temperature will affect other things on the planet that also affect the planet’s temperature. Suppose that we could magically change the composition of the atmosphere enough to raise the temperature one degree if all other parts of the earth system were held fixed, and after the warming, we allowed the other parts of the earth system to react for a few years or decades. At the end of that time, what would be the total change in the earth’s temperature?.
Answers
Due to positive feedbacks amplifying the initial alteration, the Earth would eventually become a few degrees warmer than it was before human impact.
Which phrase best sums up the greenhouse effect?The Earth becomes warmer due to the greenhouse effect when heat is trapped by gases in the atmosphere. Answer choice contains the most accurate description of this procedure (E). The right response is that the planet's surface is warmed by atmospheric gases that trap heat.
The five main causes of global warming are what?power production. A large portion of world emissions are brought on by burning fossil fuels to provide heat and power.
the process of making products.
cutting down trees in forests
making use of transportation.
food production.
Building energy production.
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A particular single-celled organism uses radiant energy to fix carbon as sugars. This organism generates ATP by breaking down sugars through a process that uses oxygen. Based on this information, how should this organism be classified?
A.
anaerobic autotroph
B.
anaerobic heterotroph
C.
aerobic autotroph
D.
aerobic heterotroph
Answers
Answer:
D
Explanation:
Answer:
For edmentum and Plato the answer is B, aerobic autotroph. For the person who asked the question the answer would be C, aerobic autotroph.
Explanation:
The process of putting a recombinant dna molecule into a cell is called.
Answers
Transfection is the process of inserting a recombinant DNA molecule into a cell. Here option A is the correct answer.
Transfection is the deliberate introduction of foreign DNA into eukaryotic cells, such as animal or plant cells, using various methods such as electroporation, lipofection, or viral vectors. This process is crucial in genetic engineering, where recombinant DNA technology is used to create genetically modified organisms (GMOs) with desirable traits, such as improved crop yield, resistance to diseases, or the production of valuable proteins.
During transfection, the recombinant DNA molecule, which is a hybrid molecule made up of DNA from different sources, is delivered into the host cell's cytoplasm or nucleus. Once inside the cell, the recombinant DNA molecule can integrate into the host genome, where it can be replicated and expressed along with the host DNA.
Transfection has revolutionized the field of biotechnology and has opened up new possibilities for research and applications in medicine, agriculture, and industry. It has also raised ethical concerns about the potential risks and unintended consequences of releasing genetically modified organisms into the environment.
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Complete question:
The process of putting a recombinant DNA molecule into a cell is called.
A) Transfection
B) Translation
C) Replication
D) Transcription
Which respiratory complication may occur if the patient is bedridden for a long duration
Answers
The respiratory complication that may occur if the patient is bedridden for a long duration is Pneumonia.
Pneumonia is a lung infection that results in inflammation of the alveoli, which are tiny air sacs in the lungs. Pneumonia can be caused by bacteria, viruses, or fungi, and it can be serious in some cases. In people who are bedridden for a long time, pneumonia is a common respiratory complication.
Inadequate ventilation, which can result in respiratory issues, is caused by a lack of movement or mobility. The lung's airways become more vulnerable to infection when a person is bedridden for an extended period. Because of the accumulation of secretions and the inability to clear them, this can result in atelectasis (collapsed lung tissue).
Pneumonia is a severe lung disease that can cause inflammation and fluid buildup in the lungs, making it difficult to breathe. To avoid respiratory complications such as pneumonia, patients who are bedridden for an extended period should have a breathing exercise routine.
It is advised that they change their positions every few hours and walk around if they are able. Furthermore, proper nutrition and hydration are critical for the body to fight off infections.
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A normal polypeptide includes the following sequence of amino acids. (Note: Each
amino acid is named by a three letter abbreviation.)
Iso-Leu-Pro-Val-His-Ser-Thr-Met
After a mutation, the amino acid sequence becomes the following:
Iso-Leu-Pro-Val-Tyr-Arg-Iso-Gly
Which of the following changes to a nitrogenous base in mRNA is most likely to
have occurred?
Answers
The messenger RNA (mRNA) is not found here but it is possible to find the correct amino acid sequence by using the genetic code.
What is the genetic code?The genetic code can be defined as a series of instructions to convert a DNA sequence to a protein sequence.
The mRNA is composed of triplets of nucleotides called codons that specify different amino acids during translation.
In conclusion, the mRNA sequence is not found here but it is possible to find the correct amino acid sequence by using the genetic code.
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photosynthesis cycles matter and energy in many environments. explain how chemosynthesis cycles matter and energy in extreme environments
Answers
Chemosynthesis enables life in light-deprived environments, utilizing chemical energy to transform inorganic substances into organic matter, sustaining organisms in extreme conditions where photosynthesis is not possible.
Photosynthesis is a natural process that converts carbon dioxide and water into oxygen and glucose by using energy from sunlight, while chemosynthesis is a process of converting inorganic substances, such as hydrogen sulfide or methane, into organic matter using energy from chemical reactions.
Chemosynthesis cycles matter and energy in extreme environments in the following ways: Energy transformation: The transfer of energy from chemical bonds to other chemical compounds allows for the creation of new life forms in environments that have no access to sunlight.
Matter transformation: Chemosynthesis helps in transforming inorganic substances, such as sulfur, into organic matter. This allows certain bacteria to survive in extreme environments, such as hydrothermal vents and caves, where no other life forms can survive.
Since chemosynthesis relies on chemical energy, it can occur in extreme environments where there is no sunlight. It is an important process that enables life forms to survive in environments where photosynthesis is impossible. For example, bacteria that undergo chemosynthesis live in deep-sea hydrothermal vents, where there is no sunlight but plenty of chemical energy from volcanic activity.
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What structural features are found in activators and what role to they play in transcription activation
Answers
Activators are proteins that bind to specific DNA sequences in the promoter region of genes and stimulate transcription initiation by RNA polymerase. They contain various structural features that enable them to interact with other proteins and DNA, ultimately leading to transcription activation.
One of the key structural features found in activators is the DNA binding domain, which allows the protein to recognize and bind to specific sequences in the promoter region. Activators may also contain activation domains, which are regions that interact with other transcriptional machinery, such as co-activators and general transcription factors, to promote transcription initiation. These domains may contain specific motifs, such as acidic or glutamine-rich sequences, that facilitate protein-protein interactions.
In addition, activators may have regulatory domains that allow for control of their own activity or interaction with other regulatory factors. These domains may include phosphorylation sites or ligand-binding domains that can modify the activator's ability to interact with other proteins or DNA.
The structural features found in activators are critical for their ability to stimulate transcription initiation. These features allow activators to bind to specific DNA sequences, interact with other transcriptional machinery, and regulate their own activity, ultimately leading to the activation of gene expression.
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Daniel expanded the expression as shown. Negative 2 (Negative 8 x minus 4 y + three-fourths) = negative 10 x minus 8 y minus 1 and one-fourth What errors did he make? Select three options. The first term should be positive. The second term should be positive. The last term should be Negative 1 and one-half, not Negative 1 and one-fourth. He divided Negative 8 by Negative 2 instead of multiplying Negative 8 by Negative 2. He did not simplify the expression completely.
Answers
Answer:
1st option, 2nd option, and third option.
Explanation:
Did this problem and got it right with these answers! :)
Answer:
a, b, and c
Explanation:
it really surprised me. trust me I'm not lying. I promise
The United States generally uses the English system of measurement. Name two items you might purchase in a grocery store that are sold in metric (SI) units. Include the SI unit used for this product? answer 3-4 sentences
Answers
Answer: Two items you might purchase in a grocery store that are sold in metric units are bread and boxes of cereal.
Explanation:
Which biome is found at the equator on every continent? A. Tropical Rainforests B. taiga/coniferous forests D. Mediterranean/chaparral
Answers
D.
Mediterranean/chaparral
All of the following conditions are associated with a deficiency of vitamin D EXCEPT ______.
-beriberi
-osteoporosis
-osteomalacia
-rickets
Answers
Vitamin D insufficiency is not linked to beriberi. People with thiamin deficiency in humans develop the sickness.
What Is beriberi caused by vitamin D deficiency?Loss of bone density brought on by a vitamin D deficiency can increase the risk of fractures and osteoporosis. Other illnesses can also be brought on by severe vitamin D deficiency. It may lead to rickets in kids. The rare condition rickets makes bones brittle and susceptible to fracture.Thiamine pyrophosphate, the biologically active form of the vitamin thiamine (also spelled thiamin), or vitamin B-1, is what is needed to treat beriberi, also known as thiamine deficiency.A lack of vitamin B1, commonly known as thiamine deficiency, results in the illness beriberi. People who consume mostly white rice or other highly refined carbohydrates in their diets frequently develop it in developing nations.To Learn more About beriberi refer To:
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Please help me I am willing to PAY YOU!! I have another question page for this if you’re able to please dm me
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A solid waste (garbage) transfer facility that sorts and breaks down garbage into recyclables and waste to be thrown away in the landfill would be a suitable analogy for which cellular organelle?
a. smooth ER
b. rough ER
c. lysosome
d. nucleus
e. mitochondrion
Answers
Answer:
Lysosomes
Explanation:
The lysosomes if the cellular organelle that is best used for the waste removal because they help in the decomposition of waste in the landfills. After the research on lysosomes, the scientists were successful in finding a bacteria that helps in decomposition of plastic waste because most of the waste is plastic which is very hard to be decomposed.
In the nutshell, lysosomes were used tens years ago but now the technological advances will refine the methods and bring ahead methods that are very efficient and cost effective as well.
Hi, could someone tell me what should be in the blank space of my concept map about the cell membrane?
Answers
when the brain's danger-detection system becomes too active, the risk for _____ disorder(s) does not increase.
Answers
when the brain's danger-detection system becomes too active, The risk for any mental health disorder does not increase when the brain's danger-detection system becomes too active.
This is because the danger-detection system is part of the brain's normal functioning, and its overactivity does not necessarily indicate the presence of a mental health disorder. The danger-detection system is responsible for detecting and responding to threats in the environment, and when it is functioning optimally, it helps us to stay alert and safe.
When it is overly active, however, it can lead to feelings of anxiety and fear. While these feelings can be uncomfortable, they do not necessarily indicate the presence of a mental health disorder. It is important to note that while the risk for a mental health disorder does not increase when the brain's danger-detection system is overly active, it can still be a sign of an underlying problem.
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5 What will MOST likely happen to the Alps millions of years in the future?
(A) The Alps will continue to be folded and grow in size.
(B) The Alps will stop folding and will get smaller in size.
(C) The Alps will eventually stop folding and remain the same size.
(D) The Alps will continue to be folded but will remain the same size.
Answers
Answer:
I don't know but im pretty sure its either C or B goodluck
Please help with this!
Answers
If it has a capital letter anywhere in the genotype that means it will be the dominant trait. If it has two lower case it’s the recessive.
Exercise promotes the insulin-independent uptake of glucose in working skeletal muscles. Given this, regular exercise would most likely reduce blood glucose levels in patients with which type(s) of diabetes?
A.Type 1 only
B.Type 2 only
C.Both Type 1 and Type 2
D.Neither Type 1 nor Type 2
Answers
Regular exercise would most likely reduce blood glucose levels in patients with B: Type 2 diabetes only.
Regular exercise promotes the insulin-independent uptake of glucose in working skeletal muscles. In Type 2 diabetes, the body's cells become resistant to insulin, which hinders the uptake of glucose from the bloodstream. However, during exercise, the muscles can still take up glucose without the need for insulin, leading to a reduction in blood glucose levels.
In contrast, Type 1 diabetes is characterized by a lack of insulin production, and exercise does not significantly impact the body's ability to produce insulin. Therefore, regular exercise is more effective in lowering blood glucose levels in patients with Type 2 diabetes compared to Type 1 diabetes.
Option B is answer.
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Write a summary statement for saturated fats including whether they are solids or liquids at room temperature and whether they have all single carbon-to-carbon bonds or at least one double carbon-to-carbon bond.
Answers
Saturated fats are usually known for being solids at room temperature and have all single carbon-to-carbon (C-C) bonds.
Give a brief account on saturated fats.Saturated fat is a type of dietary fat. Along with trans fatty acids, it is one of the fats that are bad for the body. Foods such as butter, palm oil, coconut oil, cheese, and red meat are high in saturated fat.
They are densely packed fats that are solid at room temperature and are called saturated fats because they do not contain double bonds in their chemical structure. This is because the structure contains as many hydrogen atoms as possible and is "saturated" with hydrogen. Saturated fatty acids have no double bonds between individual carbon atoms, while unsaturated fatty acids have at least one double bond in the fatty acid chain. Saturated fats are typically solid at room temperature and are of animal origin, while unsaturated fats are typically liquid and of plant origin.
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Answer:
Saturated fats are solids at room temperature and have all single carbon-to-carbon bonds.
Explanation:
edge
what is science ? explain .
Answers
Answer:
well in my view science is a technology without it we hadn't be sucess
What is the term for a female reproductive cell?
sperm
egg
tetrad
embryo
Answers
Answer:
Second choice; egg
Explanation:
Gametes are an organism's reproductive cells.
- Female gametes are called ova or egg cells
- Male gametes are called sperm
A tetrad consists of four chromatids, and a chromatid is one half of a duplicated chromosome.
An embryo is an unborn offspring (child) developing.
Answer:
Egg
Explanation:
The egg also known as ovum is the female reproductive cell.