3. how could a southern blot be used to determine if a given dna sequence contains methylated cpgs
Answers
A Southern blot can be used to determine if a given DNA sequence contains methylated CpGs by following these steps:
1. Extract and isolate the DNA from the sample you want to analyze.
2. Digest the DNA using restriction enzymes that are sensitive to methylation status. For example, use an enzyme that cuts at unmethylated CpG sites and another enzyme that cuts regardless of methylation status.
3. Separate the digested DNA fragments by size using agarose gel electrophoresis.
4. Transfer the separated DNA fragments onto a nylon or nitrocellulose membrane, maintaining the same order and distances as in the gel. This process is called "blotting."
5. Prepare a specific DNA probe that will hybridize with the target sequence containing the CpG sites of interest.
6. Label the DNA probe with a radioactive or fluorescent tag to make it detectable.
7. Hybridize the labeled probe to the DNA fragments on the membrane.
8. Wash away any unbound or non-specifically bound probes.
9. Detect the hybridized probe on the membrane using autoradiography or a fluorescence scanner. This will reveal the presence and size of DNA fragments containing the target sequence with methylated CpGs.
If the target sequence contains methylated CpGs, you will see a distinct banding pattern on the membrane that corresponds to the DNA fragments cut by the methylation-sensitive and -insensitive restriction enzymes. This allows you to determine if the given DNA sequence contains methylated CpGs.
To learn more about southern blotting visit the link below:
https://brainly.com/question/13258872
#SPJ11
Related Questions
Select ALL of the correct answers.
Which three descriptions or examples can be classified as endocrine control?
vasopressin acts on renal cells to maintain the body's water and salt balance
regulates the body using electrical signals
a reflex causes the body to move in response to a stimulus
aldosterone causes the kidneys to reabsorb water
regulates the body using hormones
a signal initiates the contraction of a muscle
Answers
Answer:
vasopressin acts on renal cells to maintain the body's water and salt balance
aldosterone causes the kidneys to reabsorb water
regulates the body using hormones
Explanation:
if i pick all of them i see which ones are right and which ones are wrong, your welcome :)
The three descriptions that can be classified as endocrine control are :
Vasopressin acts on renal cells to maintain the body's water and salt balance ( A ) Aldosterone causes the kidneys to reabsorb water ( D ) Regulates the body using hormones ( E )Endocrine control is the process of controlling, mood, growth and development by the endocrine hormones. The amount of endocrine hormones produced is dependent on the amount of hormones present already in the system of an individual and it is regulated by the endocrine system.
Some of the functions performed by the Endocrine system includes the regulation of body using hormones, aldosterone which is an endocrine hormone causing kidneys to reabsorb water.
Hence we can conclude that the three descriptions of endocrine control is as listed above.
learn more : https://brainly.com/question/2612788
Which of the following scenarios would MOST LIKELY lead to a change in DNA that would increase genetic variation in the individual’s offspring?
A) An A - T base pair changes to a C - G base pair during mitosis in a liver cell of a young man.
B) A G - C base pair changes to an A - T base pair randomly in a young woman's egg cell during meiosis.
C) UV radiation mutates the skin cells of a pregnant mother, changing two G bases to T bases.
D) A virus inserts nine base pairs into a lung cell of an elderly man.
Answers
The scenario that would likely lead to a change in DNA that would increase genetic variation in the individual’s offspring is: A G - C base pair changes to an A - T base pair randomly in a young woman's egg cell during meiosis. Option B.
What is meant by genetic variation?Genetic variation is the variance in DNA sequences among members of a population. Germ cells, such as sperm and eggs, as well as somatic (all other) cells, are subject to variation.
Genes take on various shapes, or alleles, as a result of genetic variation. People with blue eyes, for instance, have one allele of the eye color gene, whereas those with brown eyes will have a different allele of the gene.
Read more on meiosis here:https://brainly.com/question/29480363
#SPJ1
PLEASE ANSWER QUICK!!
Answers
Answer:
1, 2 and 4
Explanation:
I think they are right but im not sure, i hope it help
When you crack the back of a minion and then shake, they will glow like a glow stick. Kevin was wondering if temperature has any effect on how brightly they can glow. To test this, he will place one glowing minion in a walk-in refrigerator, one in the living room, and one in a sauna. He will then observe the brightness of each glowing minion. What is the independent or manipulated variable?
A. temp
B. brightness
C. shaking time
D. cracking the back of the minion
Answers
An enzyme is a ______ that brings about chemical reactions in living things.
Answers
AN ENZYME IS A BIOLOGICAL CATALYST !
PLEASE MARK ME BRAINLIEST!
HELP PLEASE I HAVE A WEEK LEFT OF SCHOOL I DONT WANNA FAIL
Answers
Answer:
I am too lazy to do this, but I am assuming you were supposed to do this in class. I will give you ideas.
Why is soil important? Soil is our life support system. Soils provide anchorage for roots, hold water and nutrients. Soils are home to myriad micro-organisms that fix nitrogen and decompose organic matter, and armies of microscopic animals as well as earthworms and termites.
What is soil erosion? Soil erosion is a naturally occurring process that affects all landforms. In agriculture, soil erosion refers to the wearing away of a field's topsoil by the natural physical forces of water (Figure 1) and wind (Figure 2) or through forces associated with farming activities such as tillage.
what human activities are responsible for soil erosion happening at advance rates? Ploughing, unsuitable agricultural practices, combined with deforestation and overgrazing, are the main causes of human-induced soil erosion
What are some effects on soil loss? The effects of soil erosion go beyond the loss of fertile land. It has led to increased pollution and sedimentation in streams and rivers, clogging these waterways and causing declines in fish and other species. And degraded lands are also often less able to hold onto water, which can worsen flooding.
What can be done to prevent soil erosion? Maintaining a healthy, perennial plant cover.
Mulching.
Planting a cover crop – such as winter rye in vegetable gardens. ...
Placing crushed stone, wood chips, and other similar materials in heavily used areas where vegetation is hard to establish and maintain.
Explanation: Hope this helps and have a great day
a structure that allows the middle ear to communicate with the nasopharynx is the
Answers
The structure that allows the middle ear to communicate with the nasopharynx is the Eustachian tube.
The Eustachian tube, also known as the auditory tube, is a narrow tube that connects the middle ear to the nasopharynx, which is the upper part of the throat behind the nose. The Eustachian tube plays a vital role in equalizing the air pressure in the middle ear with the outside atmosphere, which is essential for maintaining the normal functioning of the middle ear.
When the air pressure in the middle ear is different from the outside atmosphere, such as during changes in altitude or air pressure, the Eustachian tube opens briefly to allow air to flow in or out of the middle ear. This helps to equalize the pressure and prevent damage to the delicate structures of the middle ear, including the eardrum and the ossicles.
The Eustachian tube also helps to drain any fluids or mucus that may accumulate in the middle ear, preventing the buildup of pressure that can lead to ear infections.
In conclusion, the Eustachian tube is a structure that connects the middle ear to the nasopharynx, allowing for the equalization of air pressure in the middle ear and the drainage of fluids and mucus. Dysfunction of the Eustachian tube can lead to a variety of ear problems, including ear infections, hearing loss, and tinnitus. Therefore, it is important to maintain the health and function of the Eustachian tube to ensure normal middle ear function.
To know more about Eustachian tube ,visit:
https://brainly.com/question/24222218
#SPJ11
in one lab, a gene from tomato that encodes for a protein that increases heat tolerance is transformed into a yeast host. in the same lab, a gene from cows that encodes for a protein that produces a milk protein is transformed into a bacterial host. which would be considered a transgene?
Answers
In this scenario, the gene from a tomato that encodes for a protein that increases heat tolerance, which is transformed into a yeast host, would be considered a transgene. A transgene refers to a gene or DNA sequence that has been introduced into an organism from a different species through genetic engineering techniques. In this case, the tomato gene is being transferred into a non-tomato organism (yeast), making it a transgene.
On the other hand, the gene from cows that encodes for a protein that produces a milk protein and is transformed into a bacterial host would not be considered a transgene. This is because the gene is being transferred within the same species, albeit from a different individual (cow) to the bacterial host. The gene from cows is being introduced into a compatible host within its species, making it an example of genetic variation rather than a transgene.
Learn more about Transgene here
https://brainly.com/question/13062990
#SPJ11
FILL IN THE BLANK. natural populations exhibit significant phenotypic and ___ variation
Answers
Natural populations exhibit significant phenotypic and genetic variation variation
Natural populations consist of individuals who vary in their physical characteristics (phenotypes) and the underlying genetic makeup (genotypes) that determine these traits. This variation is the result of both genetic and environmental factors and is essential for the survival and adaptation of populations to changing environments. Phenotypic variation refers to differences in observable traits, such as size, shape, and behavior, while genetic variation refers to differences in the genetic information that underlies these traits. Thus, natural populations exhibit significant phenotypic and genetic variation, which is the basis for the diversity and evolution of species over time.
Learn more about genetic variation here:
https://brainly.com/question/4735157
#SPJ4
The field of biology that studies the diversity of organisms and
their evolutionary relationships is called systematics.
Select one:
True
False
.......................................................
Answers
Answer:
True.
Explanation:
Hope this helps!
Assume that the individuals listed are the true parents, each set of parents having had one of the four babies. Indicate the one family to which each of the following children belongs on the basis of blood type. Explain your reasoning.
Answers
The parents of four babies were determined using blood typing. Four sets of parents were tested for blood type and the results are as follows:
BABY | PARENT 1 | PARENT 2A | AB | OB | A | BO | AB | O | A | OA | O | BA | AB | OA:
Baby A can have one of two possible parents; either Parent 1 with blood type A, or Parent 2 with blood type O.
A's blood type is AB, which is possible only if one parent has A blood type and the other parent has B blood type. Since both Parent 1 and Parent 2 do not have B blood type, none of them can be A's parent. Thus, Baby A must have been switched with another baby in the hospital.B: Baby B can have one of two possible parents; either Parent 1 with blood type B, or Parent 2 with blood type O. B's blood type is O, which is possible only if both parents have O blood type. Since Parent 2 has O blood type, they must be Baby B's parent.
C: Baby C can have one of two possible parents; either Parent 1 with blood type AB, or Parent 2 with blood type O.
C's blood type is A, which is possible only if one parent has A blood type and the other parent has either AB or B blood type. Since Parent 1 has AB blood type, they must be Baby C's parent.D: Baby D can have one of two possible parents; either Parent 1 with blood type O, or Parent 2 with blood type A. D's blood type is O, which is possible only if both parents have O blood type. Since Parent 1 has O blood type, they must be Baby D's parent.
To know more about blood type visit:
https://brainly.com/question/27488784
#SPJ11
do eukaryotes have faster DNA replication than prokaryote?
Answers
Answer:
No
Explanation:
Replication happens at a much faster rate in prokaryotes than in eukaryotes.
Pain relievers, anti-inflammatories, antivirals, and antihistamines all manage what
Answers
Pain relievers, anti-inflammatories, antivirals, and antihistamines all manage: inflammation.
What is inflammation?An indication of inflammation may occur when a wound grows, gets red, and aches. The body's immune system reacts to an irritant by causing inflammation, to put it very simply. A splinter in the finger or other foreign item, such as a bacterium, might possibly be the source of the irritation.
Aulus Cornelius Celsus, a Roman physician, wrote about the four primary symptoms of inflammation in the first century AD. These symptoms include redness (Latin rubor), heat (calor), swelling (tumor), and pain (dolor).
Science has shown that persistent, low-grade inflammation can become a silent killer that fuels type 2 diabetes, cancer, cardiovascular disease, and other illnesses.
Thus, pain relievers, anti-inflammatories, antivirals, and antihistamines all manage: inflammation.
To know more about inflammation refer to:
https://brainly.com/question/12885835
#SPJ1
what is the tm of the most gc-rich dna sample?
Answers
The melting temperature (Tm) of the most GC-rich DNA sample will be higher than that of a less GC-rich sample.
The melting temperature, or Tm, of DNA is the temperature at which half of the DNA strands in a double-stranded DNA molecule become separated, or denatured, into single strands.
The Tm is influenced by several factors, including the length and sequence of the DNA, as well as environmental conditions such as pH and salt concentration. However, one of the most significant factors affecting Tm is the GC content of the DNA.
GC base pairs form three hydrogen bonds, which are stronger than the two hydrogen bonds formed by AT base pairs. Therefore, the higher the GC content, the more stable the DNA molecule and the higher the Tm. In general, the Tm of DNA increases by approximately 0.5-1.0°C for every 1% increase in GC content.
Therefore, the Tm of the most GC-rich DNA sample will be higher than that of a less GC-rich sample, reflecting the greater stability of the double-stranded DNA molecule due to the increased number of GC base pairs.
To know more about DNA, refer here:
https://brainly.com/question/21992450#
#SPJ11
why is newtons cradle (newtons balls) described as an “almost-ideal” closed system? explain your reasoning in 1 to 2 sentences.
Answers
The reason that newtons cradle (newtons balls) described as an “almost-ideal” closed system is because Due to the little forces that are exerting themselves onto it, it is a closed system. solely air resistance and gravity
What is the newtons cradle about?
Tn regards to the above case, it is a system that is almost completely isolated from its surroundings, Newton's cradle is referred to be an almost ideal closed system.
This indicates that the system is largely self-contained and does not interact with the environment to exchange matter or energy.
In Newton's cradle, a set of metal balls are suspended from a frame as the system. The other balls swing back and forth when one ball is pushed back and released, striking them in the process.
Therefore, The balls do not gain or lose energy during the impact since this motion is nearly completely elastic. The system is therefore almost closed and does not exchange energy with its surroundings.
Learn more about newtons cradle from
https://brainly.com/question/17696764
#SPJ1
a number of enzymes in the cytoplasm generate nadh, providing reducing power critical for electron transport and oxidative phosphorylation. but nadh does not cross the inner mitochondrial membrane and hence the electrons it provides must somehow be delivered to the matrix. this is achieved by two important shuttle systems, the ____ shuttle, and the ____ shuttle, found in muscle cells. in one of these shuttles, the electrons from nadh are transferred to ____ , and the product of this reaction, ____ is transported across the inner mitochondrial membrane, where nadh is regenerated by conversion of ____ .
Answers
The number of enzymes in the cytoplasm generate NADH, providing reducing power critical for electron transport and oxidative phosphorylation.
But NADH does not cross the inner mitochondrial membrane, and hence the electrons it provides must somehow be delivered to the matrix.
This is achieved by two important shuttle systems, the malate-aspartate shuttle, and the glycerol phosphate shuttle, found in muscle cells. In one of these shuttles, the electrons from NADH are transferred to malate, and the product of this reaction, oxaloacetate, is transported across the inner mitochondrial membrane, where NADH is regenerated by the conversion of malate.
To know more about Oxidative phosphorylation please visit :
https://brainly.com/question/8562250
#SPJ11
bioflix activity how neurons work conduction of an action potential
Answers
The transmission of an action potential in a neuron is animated in the BioFlix Activity "How Neurons Work." It demonstrates how electrical and chemical signals used by neurons to interact with one another affect the brain and other body functions.
You might need to sign in to Pearson's Channels platform, which houses BioFlix, in order to view the activity. After logging in, you should be able to find the activity by searching for "BioFlix Activity: How Neurons Work -- Conduction of an Action Potential" and accessing it right away.
As an alternative, you might discover the activity on other instructive websites. It may give anybody interested in the subject a useful visual picture of how neurons function. It is made to be used as a teaching tool for students learning about neuroscience.
To learn more about BioFlix Activity here
https://brainly.com/question/30227146
#SPJ4
Q- Bioflix activity how neurons work conduction of an action potential.
In 1907, Dr. Duncan MacDougall performed a series of experiments in which he attempted to measure the weight of the soul as it left a dying person. In his experiments, MacDougall placed a dying person on a scale and measured their weight immediately prior to and following death. MacDougall determined the change in weight to be approximately 21 grams. From these experiments, he concluded that the soul exists and has mass. What is the fundamental scientific flaw in his conclusions? (5 points)
They are based on the concept of the soul, which is beyond the bounds of science.
They are based on the idea that the soul is made of matter, not energy.
They are based on the assumption that the soul does not remain in the body after death.
They are based on the concept that the soul can be measured.
Answers
Answer:
I think so..
A. They are based on the concept of the soul, which is beyond the bounds of science.
Answer:
A. They are based on the concept of the soul, which is beyond the bounds of science.
Explanation:
Good luck! Hope this helped :)
20. Fill in the blank: When a protein is transported out of the cell, the pathway is Rough Endoplasmic Reticulum -> Vesicle -> -> Vesicle -> Cell Membrane A) Mitochondria B) Golgi Apparatus C) Lysosome D) Smooth Endoplasmic Reticulum
Answers
Answer:
B-golgi apparatus
Explanation:
This is because Golgi apparatus are used to transport substances such as secretions and protein out of the cell as well as also being involved in synthesis of lysosomes
The thymus gland is located within the mediastinum, ________ to the heart and ________ to the sternum.
Answers
Answer:
superior; posterior
Humans fishing in the ocean is an example of an abiotic factor that can negatively or positively impact the biodiversity of coral reefs. question 11 options: true false
Answers
Humans fishing in the ocean is not an example of an abiotic factor that can impact the biodiversity of coral reefs. Abiotic factors are non-living factors that affect ecosystems, such as temperature, sunlight, water pH, and nutrient levels. Therefore it is False.
The activity of humans fishing in the ocean is an example of a biotic factor, specifically an anthropogenic (human-caused) factor. Fishing practices can have both negative and positive impacts on coral reef biodiversity.
Negative impacts can occur through overfishing, where certain fish species are heavily targeted, leading to their depletion. This can disrupt the balance within the ecosystem and affect the population dynamics of coral reef species. Additionally, destructive fishing practices, such as using dynamite or cyanide, can damage coral reefs directly.
On the other hand, fishing can also have positive impacts on coral reef biodiversity through sustainable and responsible practices. Implementing regulations, such as catch limits and protected areas, can help conserve fish populations and preserve the health of coral reefs. Additionally, sustainable fishing practices can promote the conservation of marine ecosystems and the preservation of biodiversity.
In summary, while human fishing activities can have both negative and positive impacts on coral reef biodiversity, they are considered a biotic factor rather than an abiotic factor.
For more information on biotic factor visit:
brainly.com/question/27430655
#SPJ11
Good Morning. . . Ohayo. . .Please Answer this Question. . .
Which Part of Brain is Responsible for Dreams?
Answers
Answer: The hippocampus
Explanation:
In the brain's temporal lobe, the hippocampus has the ability to imagine, remember, and dream. In other words, the hippocampus is responsible for dreams.
In order to become a
resource scientist, which of
the following must be
obtained?
+5
A. Government funding
B. Doctoral degree
C. Appointment by the governor of a
state
ER
D. Swearing in by a federal judge
Answers
Answer:
B. Doctoral degree
Explanation:
An astronaut is planning a trip to a newly-discovered planet according to the law of universal gravitation, the astronaut weight in the new planet will be greater than his weight on earth if:
Answers
The new planet has more mass than Earth but the same radius. The mass of the astronaut will be calculated by the use of Newton's gravitational equation. The weight of the astronaut depends completely on its mass and the gravitational acceleration of the planet.
Gravitational acceleration is directly proportional to the mass of the planet and indirectly proportional to the radius of the planet. Hence, when the gravitational acceleration increases the planet's mass will increase therefore the radius will decrease. The astronaut's mass will depend on these factors.
Learn more about Gravitational acceleration, here
https://brainly.com/question/3009841
#SPJ1
which is the significance of carbon having four valence electrons?
Answers
Answer:
Carbon has four valence electrons, so it can achieve a full outer energy level by forming four covalent bonds.
Explanation:
ihope it helped
1 point
What evidence supports that organisms traveled in herds like elephants,
birds, or zebras. *
O index fossils
O fossils of many individuals of the same species together
identical fossils on widely separated continents
fossil marine animals in the desert
Answers
Answer:
Fossils of many individuals of the same species together
Giving brainliest!!!!!
View the worksheet below
Use the list of words to fill in the blank
Answers
Answer: Hmm!!!
Explanation:1: parathyroid
2:Thyroid
3: Adrenal
4:testes
5:Pitutary
6:Thymus
7:pancreas
8:ovary
1:parathyroid
2:thyroid
3:Adrenal
4:testes
5:Pitutary
6:Thymus
7:pancreas
8:ovary
whats the Location and the Structure for prestsynaptic membrane
Answers
Answer:
The presynaptic membrane is formed by the part of the presynaptic axon terminal forming the synapse and that of the postsynaptic neuron is called the postsynaptic membrane. The space between the presynaptic and postsynaptic membrane is called the synaptic cleft. These three structures together form the synapse.
Explanation:
If a cell has 24 chromosomes, how many chromosomes would each of its four daughter cells have after meiosis?1236six24
Answers
If a cell has 24 chromosomes before division, the daughter cell that divides during mitosis will have 24 chromosomes, and the daughter cell that divides during meiosis will have 12 chromosomes.
The cell will go through two rounds of cell division after meiosis, which will produce four haploid daughter cells with half as many chromosomes as the parent cell.
Every girl cell will have half of the first 46 chromosomes or 23 chromosomes. There are two sister chromatids on each chromosome. Now, the daughter cells enter the third and final meiosis phase: II meiosis
When a cell divides into two cells through the process of mitosis, each daughter cell has 24 chromosomes. Mitosis is a course of division of cells where a parent cell separates into two little girl cells every one of which is hereditarily very much like the parent cell.
To learn more about meiosis here
https://brainly.com/question/29383386
#SPJ4
I am offering 45 points, because I really need a good, complete answer. As a result of your Zombie Apocalypse, the grass at a local park will no longer be mowed. What type of succession is this (primary or secondary)? Describe the stages of succession that will occur in the next 100 years.
Answers
Answer: the next 100 years won't matter as long as you have a good soil because it contains a good amount of nutrients for farming other than that I don't know what to say I can't really understand your question
Explanation: